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Furthermore, autophagy is closely related to ERS under apoptosis. Here, this study aimed to investigate the role of the reciprocal interaction between autophagy and ERS in the cigarette smoke extract (CSE)\u2010induced apoptosis of human bronchial epithelial (HBE) cells. Cell apoptosis was detected by flow cytometry analysis. Protein expression was examined by Western blot. The mRNA expression was detected using real\u2010time quantitative reverse transcription PCR (qRT\u2010PCR). The results showed that CSE treatment induced apoptosis, autophagy, and expression of ERS\u2010related proteins in HBE cells. Furthermore, autophagy inhibition by 3\u2010MA significantly decreased protein expression of GRP78, p\u2010PERK, and p\u2010eIF2\u03b1 and increased CHOP, ATF4, and caspase\u20104, whereas ERS inhibition by 4\u2010PBA led to autophagy suppression. Moreover, the CSE\u2010induced autophagy was diminished by knockdown of GRP78, PERK, or eIF2\u03b1 but enhanced by knockdown of ATF4 or CHOP; however, the CSE\u2010induced HBE apoptosis was enhanced by knockdown of GRP78, PERK, or eIF2\u03b1 but was attenuated by knockdown of ATF4 or CHOP. Additionally, both sodium hydrosulfide (NaHS) and melatonin attenuated the CSE\u2010induced apoptosis, enhanced the CSE\u2010induced autophagy, increased GRP78, p\u2010PERK, and p\u2010eIF2\u03b1, and decreased CHOP, ATF4, and caspase\u20104, via SIRT1\/ORP150 pathway. Collectively, this study provided evidence about the role of the reciprocal interaction between autophagy and ERS in CSE\u2010induced apoptosis of HBE cells. \u00a9 2018 IUBMB Life, 71(1):66\u201380, 2019<\/jats:p>","DOI":"10.1002\/iub.1937","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T21:26:59Z","timestamp":1539811619000},"page":"66-80","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Role of reciprocal interaction between autophagy and endoplasmic reticulum stress in apoptosis of human bronchial epithelial cells induced by cigarette smoke extract"],"prefix":"10.1002","volume":"71","author":[{"given":"Baimei","family":"He","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, China; Institute of Clinical Pharmacology Central South University, Hunan Key Laboratory of Pharmacogenetics  Changsha 410078 China"},{"name":"Department of Geriatric Medicine, Xiangya Hospital Central South University  Changsha Hunan Province China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University  Changsha Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Geriatric Medicine, Xiangya Hospital Central South University  Changsha Hunan Province China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University  Changsha Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongbo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Geriatric Medicine, Xiangya Hospital Central South University  Changsha Hunan Province China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University  Changsha Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Geriatric Medicine, Xiangya Hospital Central South University  Changsha Hunan Province China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University  Changsha Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0154-921X","authenticated-orcid":false,"given":"Zhaoqian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, 410008, China; 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