{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:52:54Z","timestamp":1760953974850,"version":"3.40.5"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T00:00:00Z","timestamp":1538697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific Research Fund of Chinese Medicine of Shanghai Municipal Health Bureau","award":["2012J021A"],"award-info":[{"award-number":["2012J021A"]}]},{"name":"Key Discipline Construction Project of Pudong Health Bureau of Shanghai","award":["PWZxk2017-23"],"award-info":[{"award-number":["PWZxk2017-23"]}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gliomas are the most commonly occurring primary malignant brain tumors in the central nervous system of adults. They are rarely curable and the prognosis for high grade gliomas is generally poor. Recently, long non\u2010coding RNA (lncRNA) human ovarian cancer\u2010specific transcript 2 (HOST2) has been reported to be expressed at high levels in human ovarian cancer, involving tumorigenesis. However, little is still known about whether and how HOST2 regulates glioma development and progression. Therefore, this study aims to investigate the role of HOST2 in human glioma cells. Reverse transcription quantitative real\u2010time polymerase chain reaction (RT\u2010qPCR) was used to determine the expression of lncRNA HOST2, let\u20107b, and PBX3 in human glioma cells. Cultured human glioma cells were treated with siRNA (si)\u2010lncRNA HOST2, let\u20107b mimic, si\u2010lncRNA HOST2 + let\u20107b inhibitor, and si\u2010PBX3. Parameters including cell viability, colony formation, cell migration, and cell invasion were detected by cell counting kit\u20108 assay, colony formation assay, scratch test, and Transwell assay respectively to determine the effects of down\u2010regulated HOST2 on glioma cells. Tumor formation in nude mice was evaluated by subcutaneous tumor formation experiment. Results showed that HOST2 and PBX3 were highly expressed in glioma tissue whereas let\u20107b was expressed at much lower levels. In response to treatment with si\u2010lncRNA HOST2, si\u2010PBX3, and let\u20107b mimic, glioma cell lines exhibited decreased cell viability, suppressed cell migration, invasion, and reduced colony formation of glioma cells. This was accompanied by an attenuated tumor formation with smaller volume and weight in nude mice, suggesting that down\u2010regulated HOST2 could inhibit the tumorigenicity of glioma cells. Lastly, we found that lncRNA HOST2 was highly expressed in glioma tissues and its down\u2010regulation could inhibit the growth and invasion of glioma cells. \u00a9 2018 IUBMB Life, 71(1):93\u2013104, 2019<\/jats:p>","DOI":"10.1002\/iub.1943","type":"journal-article","created":{"date-parts":[[2018,10,6]],"date-time":"2018-10-06T10:17:17Z","timestamp":1538821037000},"page":"93-104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["An <i>in vitro<\/i> and <i>in vivo<\/i> study of the role of long non\u2010coding RNA\u2010HOST2 in the proliferation, migration, and invasion of human glioma cells"],"prefix":"10.1002","volume":"71","author":[{"given":"Qi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong\u2010Wei","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Ming","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Qiang","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010Yi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Long","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3750-6438","authenticated-orcid":false,"given":"Kui\u2010Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Shanghai East Hospital Affiliated to Tongji University  Shanghai 200120 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.428"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2011.01.011"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e318271f7cb"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nou087"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1378"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.07.075"},{"key":"e_1_2_8_8_1","first-page":"591","article-title":"Application of hyperthermia induced by superparamagnetic iron oxide nanoparticles in glioma treatment","volume":"6","author":"Silva A. 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