{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T18:26:13Z","timestamp":1781893573669,"version":"3.54.5"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T00:00:00Z","timestamp":1538524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["JJ2018ZR1554"],"award-info":[{"award-number":["JJ2018ZR1554"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our earlier work has shown that the E3 ligase <jats:italic>TRIM56<\/jats:italic> messenger RNA (mRNA) level and vimentin protein expression followed an inverse correlation in ovarian carcinoma patients; however, the regulatory mechanisms underlying TRIM56 expression is unclear. Steady state expression of <jats:italic>TRIM56<\/jats:italic> mRNA expression in the normal ovarian cell line Moody and ovarian cancer cell lines SKOV\u20103, A2780, and Caov\u20103 were not significantly different; however, TRIM56 protein expression was significantly lower in the ovarian cancer cell lines compared to the Moody cell line. Polysome profiling showed significant increase in translation of <jats:italic>TRIM56<\/jats:italic> messenger RNA in the Moody cells compared to the SKOV\u20103 cells. We performed RNA\u2010affinity pulldown using biotinylated TRIM56 5 \u2018and 3\u2019\u2010UTR and postnuclear extracts from Moody and SKOV\u20103 cells. Whereas no notable difference was observed in affinity pull\u2010down obtained with the 5\u2019\u2010UTR, there was obvious difference in protein binding patterns with the 3\u2019\u2010UTR. Mass spectrometry was used to determine the most differentially binding protein as poly r (c) binding protein 1 (PCBP1). PCBP1 expression and binding to the 3\u2019\u2010UTR was both higher in SKOV\u20103 cells compared to the Moody cells. Silencing of <jats:italic>TRIM56<\/jats:italic> in Moody cells cause an increase in <jats:italic>in vitro<\/jats:italic> migration and invasion, and a similar effect was mimicked by overexpression of <jats:italic>PCBP1<\/jats:italic>. Conversely, silencing of <jats:italic>PCBP1<\/jats:italic> or overexpression of <jats:italic>TRIM56<\/jats:italic> in SKOV\u20103 cells significantly decreased <jats:italic>in vitro<\/jats:italic> migration and invasion. In xenograft assays, SKOV\u20103 cells stably overexpressing shRNA targeting <jats:italic>PCBP1<\/jats:italic> decreased metastasis, whereas shRNA\u2010targeting <jats:italic>TRIM56<\/jats:italic> potentiated detection of metastatic lesions, compared to the parental SKOV\u20103 cells themselves. Taken together our results reveal a yet undefined posttranscriptional regulatory mechanism underlying low expression of TRIM56 in ovarian cancer. \u00a9 2018 IUBMB Life, 71(1):177\u2013182, 2019<\/jats:p>","DOI":"10.1002\/iub.1948","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T17:20:47Z","timestamp":1538760047000},"page":"177-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Poly r(C) Binding Protein 1 Regulates Posttranscriptional Expression of the Ubiquitin Ligase TRIM56 in Ovarian Cancer"],"prefix":"10.1002","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4297-1337","authenticated-orcid":false,"given":"Lei","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing  Daqing Heilongjiang 163319 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010gang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing  Daqing Heilongjiang 163319 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing  Daqing Heilongjiang 163319 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010feng","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing  Daqing Heilongjiang 163319 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010jie","family":"Su","sequence":"additional","affiliation":[{"name":"College of Medical Laboratory Science and Technology Harbin Medical University at Daqing  Daqing Heilongjiang 163319 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084941"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041842"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"3506","DOI":"10.18632\/oncotarget.6510","article-title":"FOXP1 functions as an oncogene in promoting cancer stem cell\u2010like characteristics in ovarian cancer cells","volume":"7","author":"Choi E. 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