{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:03:26Z","timestamp":1781586206415,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,2]],"date-time":"2018-12-02T00:00:00Z","timestamp":1543708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"DBT-IISc partnership program"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The plant toxin, abrin, a type\u2010II ribosome inactivating protein, is extremely lethal, the human fatal dose being ~1 \u03bcg\/kg body weight. Abrin has been classified as an agent for bioterrorism, which is of concern. Conversely, the high toxic property of abrin has been employed in generating immunotoxins, whereas its toxin moiety is conjugated to cell surface marker\u2010specific antibodies for cell\u2010targeted killing. Different cell types exhibit variable levels of sensitivity to abrin toxicity; therefore, adequate knowledge of the molecular mechanism that governs the activity of the protein would be a safeguard. To gain insights into this, two cell lines requiring strikingly different concentrations of abrin for inactivating ribosomes were studied. Employing conjugates of the wild\u2010type and active site mutant of abrin A chain with the ricin B chain, it was found that abrin\u2010induced apoptosis was dependent on inhibition of protein synthesis (PSI) leading to ER\u2010stress in Ovcar\u20103 cells, but not in KB cells. Abrin was also observed to cause direct DNA damage in KB cells, while in Ovcar\u20103 cells abrin\u2010induced DNA damage was found to be dependent on caspases. Overall, the study demonstrates that the correlation of abrin\u2010mediated PSI and apoptosis is cell\u2010specific and abrin can induce more than one pathway to cause cell death. \u00a9 2018 IUBMB Life, 71(3):357\u2013363, 2019<\/jats:p>","DOI":"10.1002\/iub.1980","type":"journal-article","created":{"date-parts":[[2018,12,3]],"date-time":"2018-12-03T05:15:13Z","timestamp":1543814113000},"page":"357-363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Correlation of abrin\u2010mediated inhibition of protein synthesis and apoptosis"],"prefix":"10.1002","volume":"71","author":[{"given":"Vinita","family":"Tiwari","sequence":"first","affiliation":[{"name":"Department of Biochemistry Indian Institute of Science  Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1251-7085","authenticated-orcid":false,"given":"Anjali A.","family":"Karande","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Indian Institute of Science  Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(81)90116-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0492-405"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43000-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058304"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20030797"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2010.08.012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kft139"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100571200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-015-2355-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0521com"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092586"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20131110"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21803"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.05.003"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.01.038"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40880-015-0030-x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728220902980249"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0524-2"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.07.085"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.3.518"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb19917.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3190507"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fiub.1980","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.1980","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T11:48:27Z","timestamp":1694692107000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.1980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,2]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/iub.1980"],"URL":"https:\/\/doi.org\/10.1002\/iub.1980","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,2]]},"assertion":[{"value":"2018-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}