{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:48Z","timestamp":1786977888750,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Osteoarthritis (OA) is a degenerative joint disease characterized by joint pain, stiffness, and function degeneration with high incidence. Recent studies have been inspired based on the association between microRNAs (miRs) and therapeutic research of OA. Hence, the present study evaluates the effects of miR\u2010138 on chondrocyte proliferation, differentiation, and apoptosis through the WNT\/\u03b2\u2010catenin signaling pathway in mice with OA by binding to NIMA\u2010related kinase 2 (NEK2). Appropriate dataset was selected from the Gene Expression Omnibus database, and differentially expressed genes and potential miRNAs that could regulate NEK2 were explored. A mouse model of OA was established. The expressions of miR\u2010138, NEK2, \u03b2\u2010catenin, GSK3\u03b2, Bcl\u20102, Bcl\u20102\u2010associated X protein (Bax), p53, MMP\u201013, Col2, and Aggrecan and the phosphorylation levels of \u03b2\u2010catenin were determined by the reverse transcription quantitative polymerase chain reaction and Western blot analysis. The 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>H<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010tetrazolium bromide (MTT) assay and flow cytometry were employed to detect cell proliferation and apoptosis, respectively. The potential functional role of NEK2 was revealed to be related to the WNT\/\u03b2\u2010catenin signaling pathway, and miR\u2010138 was the putative regulator of NEK2. miR\u2010138 expression was downregulated while expressions of NEK2 and \u03b2\u2010catenin as well as the phosphorylation levels of \u03b2\u2010catenin were upregulated in mice with OA. The chondrocytes treated with miR\u2010138 mimic and siRNA\u2010NEK2 exhibited reduced expressions of NEK2, \u03b2\u2010catenin, MMP\u201013, Bax, and p53 and elevated expressions of Col2, Aggrecan, and Bcl\u20102 as well as phosphorylation levels of \u03b2\u2010catenin along with enhanced chondrocytes' proliferation and suppressed cell apoptosis. Overexpression of miR\u2010138 induces cell survival and reduces WNT\/\u03b2\u2010catenin signaling of OA chondrocytes through NEK2. \u00a9 2019 IUBMB Life, 71(9):1355\u20131366, 2019\n                  <\/jats:p>","DOI":"10.1002\/iub.2050","type":"journal-article","created":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T21:20:02Z","timestamp":1556659202000},"page":"1355-1366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["microRNA\u2010138 induces cell survival and reduces WNT\/\u03b2\u2010catenin signaling of osteoarthritis chondrocytes through NEK2"],"prefix":"10.1002","volume":"71","author":[{"given":"Weiling","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Radiology The First Hospital of Jilin University  Changchun People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peihong","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Radiology The First Hospital of Jilin University  Changchun People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jilin Province Population Life Science and Technology Research Institute  Changchun People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Piao","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics The First Hospital of Jilin University  Changchun People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-0208","authenticated-orcid":false,"given":"Dong","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Radiology The First Hospital of Jilin University  Changchun People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-204698"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.20344\/amp.5477"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/lds038"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-017-0428-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.611.BJR-2017-0090.R1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.510.BJR-2016-0074.R2"},{"key":"e_1_2_9_8_1","first-page":"2177","article-title":"miR\u2010138 suppressed the progression of osteoarthritis mainly through targeting p65","volume":"21","author":"Wei Z. 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