{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:14:51Z","timestamp":1747149291567,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T00:00:00Z","timestamp":1565913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study addresses the demand through datamining The Cancer Genome Atlas (TCGA) database and elucidates mechanistic involvements of interferon alpha 8 (IFNA8) in lung cancer. The overall survival and disease\u2010free survival of lung cancer patients in respect to IFNA8 expression level were analyzed. IFNA8 expression levels in both serum and tumor tissue were determined by real\u2010time polymerase chain reaction. The diagnostic value of serous IFNA8 in lung cancer was assessed by receiver operating characteristic (ROC) curve analysis. Cell viability and proliferation were measured by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide and Cell Counting Kit\u20108 assays. in vivo pro\u2010tumor effect of IFNA8 was evaluated using xenograft tumor model. The metastasis\u2010prone behaviors were determined by Transwell chamber assay and tail vein\u2010injection in mice. Protein levels of p\u2010AKT, total AKT, and endogenous reference actin were analyzed by western blot. We uncovered high IFNA8 associated with unfavorable overall survival and disease\u2010free survival in lung cancer patients from TCGA. We further characterized the aberrant over\u2010expression of IFNA8 in both peripheral blood and solid tumor from our clinical patient panel, and ROC analysis suggested its potential diagnostic value. Ectopic over\u2010expression of IFNA8 promoted viability and proliferation in both A549 and H1299 cells in vitro and accelerated xenograft tumor growth in vivo. Furthermore, IFNA8 facilitated migration, invasion, and metastasis of A549 cells in vivo. Mechanistically, we disclosed the over\u2010activation of AKT signaling in IFNA8\u2010proficient A549 cells, inhibition of which completely abolished the pro\u2010tumor effects of IFNA8. We have identified IFNA8 as a novel biomarker for either diagnostic or prognostic purpose in lung cancer, which is mechanistically associated with abnormal activation of AKT signaling.<\/jats:p>","DOI":"10.1002\/iub.2152","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T14:12:28Z","timestamp":1565964748000},"page":"1806-1814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serous IFNA3 predicts unfavorable prognosis in lung cancer via abnormal activation of AKT signaling"],"prefix":"10.1002","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8881-3465","authenticated-orcid":false,"given":"Quanxu","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Radiology Weihai Municipal Hospital  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peixia","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of General Surgery Weihai Municipal Hospital  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Healthcare Weihai Municipal Hospital  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21166"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(16)30200-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30958-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2017.0073"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4024-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.08.003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.56"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3133"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4161\/onci.19964"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-0644"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10673"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00450-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2014.05.008"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/crj.12414"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13865"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357907.2016.1223301"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32258"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2017.10.002"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2017.10.027"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2015.1116539"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18010226"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2017.12.002"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000480414"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.22660"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.292"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.10.042"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/CAD.0000000000000479"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iub.2152","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T08:08:29Z","timestamp":1694246909000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.2152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,16]]},"references-count":27,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1002\/iub.2152"],"URL":"https:\/\/doi.org\/10.1002\/iub.2152","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"type":"print","value":"1521-6543"},{"type":"electronic","value":"1521-6551"}],"subject":[],"published":{"date-parts":[[2019,8,16]]},"assertion":[{"value":"2019-06-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}