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Neuroendocrine cells release hormones and neuropeptides through calcium\u2010regulated exocytosis, a process that is coordinated by a fine remodeling of the actin cytoskeleton. We describe here that calcium\u2010regulated exocytosis is impaired in chromaffin and PC12 cells with reduced \u03b1II\u2010spectrin expression levels. Using yeast two\u2010hybrid screening, we show that neuronal Wiskott\u2013Aldrich Syndrome protein (N\u2010WASP) is a partner of the \u03b1II\u2010spectrin SH3 domain and demonstrate that secretagogue\u2010evoked N\u2010WASP recruitment at cell periphery is blocked in the absence of \u03b1II\u2010spectrin. Additionally, experiments performed with ectopically expressed \u03b1II\u2010spectrin mutant unable to bind N\u2010WASP indicated that the interaction between SH3 domain and N\u2010WASP is pivotal for neuroendocrine secretion. Our results extend the list of spectrin interactors and strengthen the idea that \u03b1II\u2010spectrin is an important scaffold protein that gathers crucial actin\u2010related players of the exocytic machinery.<\/jats:p>","DOI":"10.1002\/iub.2217","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T12:15:48Z","timestamp":1576844148000},"page":"544-552","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["\u03b1II\u2010spectrin controls calcium\u2010regulated exocytosis in neuroendocrine chromaffin cells through neuronal Wiskott\u2013Aldrich Syndrome protein interaction"],"prefix":"10.1002","volume":"72","author":[{"given":"S\u00e9bastien","family":"Houy","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Int\u00e9gratives Universit\u00e9 de Strasbourg  Strasbourg 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