{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:47:25Z","timestamp":1778600845740,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T00:00:00Z","timestamp":1581033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011470","name":"Shanghai Minhang Science and Technology Commission","doi-asserted-by":"publisher","award":["2019MHZ068"],"award-info":[{"award-number":["2019MHZ068"]}],"id":[{"id":"10.13039\/501100011470","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we intended to determine the detailed function and mechanism of long noncoding RNA (lncRNA) <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/643911\">colorectal neoplasia differentially expressed<\/jats:ext-link> (CRNDE) in liver injury induced by sepsis. Cecal ligation and perforation (CLP) models were adopted to induce sepsis in vivo with rats, and hepatic epithelial cells L02 were treated with lipopolysaccharide (LPS) to mimic sepsis in vitro. Enzyme\u2010linked immunosorbent assay was conducted to detect the levels of tumor necrosis factor (TNF\u2010\u03b1), interleukin\u20106 (IL\u20106), interleukin\u201010 (IL\u201010), and interferon\u2010\u03b3 (IFN\u2010<jats:bold>\u03b3)<\/jats:bold> in the serum of rats. Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was performed to measure the expressions of CRNDE and microRNA\u2010126\u20105p (miR\u2010126\u20105p). Flow cytometry analysis and Cell Counting Kit\u20108 (CCK\u20108) method were carried out followed by the up\u2010 or downregulation of CRNDE and miR\u2010126\u20105p to monitor the proliferation and apoptosis of L02 cells, respectively. Western blot was then applied to determine the expressions of cysteinyl aspartate specific proteinase 3 (caspase 3), poly(ADP\u2010ribose)polymerase (PARP), cytochrome <jats:italic>c<\/jats:italic>, and <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/599\">BCL2\u2010like 2<\/jats:ext-link> (BCL2L2). The interactions between CRNDE with miR\u2010126\u20105p and miR\u2010126\u20105p with BCL2L2 were determined through bioinformatics, qRT\u2010PCR, dual luciferase reporter assay, and RNA immunoprecipitation assay. CRNDE was significantly decreased in liver tissues and hepatic cells in sepsis models. Upregulation of CRNDE promoted the viability of L02 cells and inhibited their apoptosis, while downregulation of CRNDE had opposite effects. The expression of CRNDE in liver tissues of septic rats was correlated with the expression miR\u2010126\u20105p. It was also demonstrated that the transfection of miR\u2010126\u20105p mimics reversed the inhibitory effect induced by CRNDE on apoptosis of L02 cells. CRNDE could specifically bind to miR\u2010126\u20105p and reduce its expression, in turn promote the expression of BCL2L2. Additionally, CRNDE overexpression in rats ameliorated liver injury induced by sepsis. Downregulated CRNDE aggravates hepatic injury via regulating miR\u2010126\u20105p and BCL2L2 during sepsis.<\/jats:p>","DOI":"10.1002\/iub.2230","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T13:58:49Z","timestamp":1581083929000},"page":"440-451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Long noncoding RNA colorectal neoplasia differentially expressed alleviates sepsis\u2010induced liver injury via regulating miR\u2010126\u20105p"],"prefix":"10.1002","volume":"72","author":[{"given":"Yang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Emergency, Minhang Hospital, Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfeng","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zichen","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Emergency, Minhang Hospital, Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Emergency, Minhang Hospital, Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0931-3623","authenticated-orcid":false,"given":"Keyu","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Emergency, Minhang Hospital, Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053741"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.012"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.02.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.12.029"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2017.0061"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4509"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2016.71"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.04.192"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317703821"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00006.2010"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3727\/096504016X14685034103879"},{"issue":"3","key":"e_1_2_8_14_1","first-page":"3126","article-title":"Tubeimoside\u20101 inhibits the proliferation and metastasis by promoting miR\u2010126\u20105p expression in non\u2010small cell lung cancer cells","volume":"16","author":"Shi H","year":"2018","journal-title":"Oncol Lett"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-008-0300-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13817"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20170339"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40635-018-0197-y"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2422"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2013.1482"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.13049"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2018.06.008"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28894"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.168"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc11381"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.03.031"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26933"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1967"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2011.09.008"},{"key":"e_1_2_8_30_1","first-page":"5030","article-title":"Mitochondrial mechanisms of sepsis\u2010induced organ failure","volume":"13","author":"Exline MC","year":"2008","journal-title":"Front Biosci"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-007-0154-9"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.07.002"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402149"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-019-0190-4"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-019-0142-4"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2033"},{"issue":"8","key":"e_1_2_8_37_1","first-page":"3512","article-title":"Influence of lncRNA HOTAIR on acute kidney injury in sepsis rats through regulating miR\u201034a\/Bcl\u20102 pathway","volume":"23","author":"Jiang ZJ","year":"2019","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00491.x"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fiub.2230","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2230","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iub.2230","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T14:34:29Z","timestamp":1694010869000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.2230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,7]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1002\/iub.2230"],"URL":"https:\/\/doi.org\/10.1002\/iub.2230","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,7]]},"assertion":[{"value":"2019-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}