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Fluorescence recovery after photobleaching of the Z\u2010ring in live bacteria demonstrated that BT\u2010benzo\u201029 strongly suppressed the assembly dynamics of FtsZ in the Z\u2010ring. Furthermore, <jats:italic>B. subtilis<\/jats:italic> cells expressing V275A\u2010FtsZ resisted the antibacterial activity of BT\u2010benzo\u201029 providing evidence that BT\u2010benzo\u201029 inhibits bacterial proliferation by targeting FtsZ. In addition, a brief (8\u2009min) exposure of BT\u2010benzo\u201029 destroyed the Z\u2010ring without perturbing the localization of a late cell division protein, DivIVA, the nucleoid segregation, and membrane permeability. BT\u2010benzo\u201029, when used in combination with vancomycin and polymyxin B (PMB), produced a much stronger inhibitory effect on <jats:italic>Bacillus subtilis<\/jats:italic> and <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content> cells, respectively. The combination index of BT\u2010benzo\u201029 with vancomycin and PMB was determined to be &lt;1, suggesting that BT\u2010benzo\u201029 exhibits synergistic inhibitory effects on bacterial proliferation when used along with these antibiotics.<\/jats:p>","DOI":"10.1002\/iub.2234","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T02:15:36Z","timestamp":1580091336000},"page":"978-990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mechanistic insight into the effect of BT\u2010benzo\u201029 on the Z\u2010ring in <i>Bacillus subtilis<\/i>"],"prefix":"10.1002","volume":"72","author":[{"given":"Kishore","family":"Kunal","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishu","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemendra P. 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