{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T12:07:53Z","timestamp":1788005273727,"version":"build-2784847793"},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guangdong Science and Technology Project","award":["A2019416"],"award-info":[{"award-number":["A2019416"]}]},{"name":"Youth Research Cadre Cultivation Project of Shenzhen People's Hospital","award":["SYKYPY201904"],"award-info":[{"award-number":["SYKYPY201904"]}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of the present study was to investigate the molecular mechanism of miR\u2010182 in kidney fibrosis in polycystic kidney disease (PKD). We measured the expression of miR\u2010182 in kidney tissue of autosomal dominant PKD. Additionally, we investigated the relationship between miR\u2010182 and fibrotic protein by transfecting miR\u2010182 mimics and miR\u2010182 inhibitor into polycystic kidney cyst\u2010lined epithelial cells, respectively. Furthermore, we observed the interaction between transforming growth factor \u03b21 (TGF\u2010\u03b21) and miR\u2010182 and fibrinogen factors of cyst\u2010lined epithelial cells after TGF\u2010\u03b21 intervention, and measured the expression of Smad2 and Smad3 protein. Results are presented as follows: (a) MiR\u2010182 was positively correlated with fibrosis of cyst\u2010lined epithelial cells; (b) TGF\u2010\u03b21 could induce fibrosis of cyst\u2010lined epithelial cells; (c) the expression of miR\u2010182 had a remarkably impact on the fibrosis induced by TGF\u2010\u03b21, but had little effect on the expression of TGF\u2010\u03b21; (d) the expression of Smad3 protein in TGF\u2010\u03b21 induce\u2010cyst\u2010lined epithelial cells was increased. TGF\u2010\u03b21 and miR\u2010182 promoting the fibrosis of polycystic kidney cyst\u2010lined epithelial cells may be mediated by the TGF\u2010\u03b21\/Smad3 signaling pathway, of which Smad3 was an important regulator.<\/jats:p>","DOI":"10.1002\/iub.2255","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T07:26:22Z","timestamp":1582010782000},"page":"1340-1348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["MiR\u2010182 inhibits kidney fibrosis by regulating transforming growth factor \u03b21\/Smad3 pathway in autosomal dominant polycystic kidney disease"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1222-2298","authenticated-orcid":false,"given":"Liping","family":"Sun","sequence":"first","affiliation":[{"name":"Key Renal Laboratory of Shenzhen, Department of Nephrology, Shenzhen People's Hospital The Second Clinical Medical College of Jinan University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaofeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Medicine Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Renal Laboratory of Shenzhen, Department of Nephrology, Shenzhen People's Hospital The Second Clinical Medical College of Jinan University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Renal Laboratory of Shenzhen, Department of Nephrology, Shenzhen People's Hospital The Second Clinical Medical College of Jinan University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00406.2016"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-013-2706-2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12389"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mnh.0000437612.50040.ae"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3547"},{"issue":"4","key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1093\/ndt\/gft223","article-title":"MicroRNAs in kidney diseases: New promising biomarkers for diagnosis and monitoring","volume":"29","author":"Schena FP","year":"2014","journal-title":"Nephrol Dialysis Transplant: Off Publ Eur Dialysis Transplant Assoc\u2014Eur Renal 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