{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:42:10Z","timestamp":1790775730674,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cutaneous leishmaniasis is commonly caused by\n                    <jats:italic>Leishmania major<\/jats:italic>\n                    and\n                    <jats:italic>Leishmania tropica<\/jats:italic>\n                    . In the present study, the differential expression of proteins was identified in the amastigote\u2010like forms of\n                    <jats:italic>L. tropica<\/jats:italic>\n                    and\n                    <jats:italic>L. major<\/jats:italic>\n                    in Iranian isolates. Initially, the samples were cultured and identified using PCR\u2010RFLP technique. The\n                    <jats:italic>Leishmania<\/jats:italic>\n                    isolates were then grown in host\u2010free (axenic) culture and prepared to amastigote\u2010like forms, followed by the extraction of their proteins. To identify significant differentially expressed proteins (DEPs) of two types of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    , the label\u2010free quantitative proteomic technique was used based on sequential window acquisition of all theoretical fragment ion spectra mass spectrometry. A total of 51 up\/down\u2010DEPs (fold change &gt;2 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2010value &lt;.05) were identified between the axenic amastigote forms of\n                    <jats:italic>L. major<\/jats:italic>\n                    and\n                    <jats:italic>L. tropica.<\/jats:italic>\n                    Of these, 34 and 17 proteins were up\u2010regulated in\n                    <jats:italic>L. major<\/jats:italic>\n                    and\n                    <jats:italic>L. tropica<\/jats:italic>\n                    , respectively. Several enriched GO terms were identified via biological process analyses for DEPs; furthermore, the metabolic process and translation were disclosed as top category in the up\u2010regulated proteins of both\n                    <jats:italic>L. major<\/jats:italic>\n                    and\n                    <jats:italic>L. tropica<\/jats:italic>\n                    species. Also, the KEGG analysis revealed carbon metabolism and metabolic pathways term as the top pathways in the proteins up\u2010regulated in\n                    <jats:italic>L. major<\/jats:italic>\n                    and\n                    <jats:italic>L. tropica<\/jats:italic>\n                    , respectively. Taken together, the numerous novel DEPs identified between the studied species could help fully understand the molecular mechanisms of pathogenesis and provide potential drug targets and vaccine candidates.\n                  <\/jats:p>","DOI":"10.1002\/iub.2300","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T16:14:32Z","timestamp":1588263272000},"page":"1715-1724","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative proteomic analysis to determine differentially expressed proteins in axenic amastigotes of\n                    <scp>\n                      <i>Leishmania tropica<\/i>\n                      and\n                      <i>Leishmania major<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"72","author":[{"given":"Marzieh","family":"Ashrafmansouri","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology, Student Research Committee, School of Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"},{"name":"Diagnostic Laboratory Sciences and Technology Research Center, Faculty of Paramedical Sciences Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasrin","family":"Amiri\u2010Dashatan","sequence":"additional","affiliation":[{"name":"Proteomics Research Center, Faculty of Paramedical Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2243-8276","authenticated-orcid":false,"given":"Nayebali","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Proteomics Research Center, Faculty of Paramedical Sciences Shahid Beheshti University of Medical Sciences  Tehran 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