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Hence, exploring the proper treatment methods is a must in tacking this disease. qRT\u2010PCR and western blot were used to detect the expression of genes and proteins, respectively. The proliferation of human retinal vascular endothelial cells (HRECs) was ensured by MTT assay. The luciferase activity was measured through luciferase assay. The inverted phase\u2010contrast light microscope was used to observe the formation of a vascular tube. In the present study, our data demonstrated that circPDE4B was downregulated, while hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) and VEGFA were upregulated in the retinopathy of prematurity model in vitro and in vivo. CircPDE4B increasing remarkably inhibited the expression of HIF\u20101\u03b1 and VEGFA in hypoxia\u2010induced HRECs and subsequent repressed cell proliferation and pathological angiogenesis. We further found that miR\u2010181c suppressed the expression of von Hippel\u2013Lindau (VHL), while circPDE4B could promote VHL expression via binding to miR\u2010181c. Finally, our results revealed that circPDE4B inhibited the expression of VEGFA and pathological angiogenesis via facilitating VHL\u2010mediated ubiquitin degradation of HIF\u20101\u03b1. In conclusion, circPDE4B suppressed the expression of VEGFA and pathological angiogenesis via promoting VHL\u2010mediated ubiquitin degradation of HIF\u20101\u03b1 through binding to miR\u2010181c. Our study indicated that circPDE4B might be an effective therapeutic target of retinopathy of prematurity.<\/jats:p>","DOI":"10.1002\/iub.2307","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T13:48:34Z","timestamp":1593092914000},"page":"1920-1929","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<scp>CircPDE4B<\/scp> inhibits retinal pathological angiogenesis via promoting degradation of <scp>HIF<\/scp>\u20101\u03b1 though targeting <scp>miR<\/scp>\u2010181c"],"prefix":"10.1002","volume":"72","author":[{"given":"Yan","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Pediatric Ophthalmology The Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shurong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatric Ophthalmology The Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuanglian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatric Ophthalmology The Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunhong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Ophthalmology The Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Ophthalmology The Second Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Histology and 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