{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T22:02:20Z","timestamp":1794261740447,"version":"4.9.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Clinical and basic study on the abnormal expression of lysyl oxidase in elderly patients with heart failure","award":["142102310401"],"award-info":[{"award-number":["142102310401"]}]},{"name":"Clinicaleffect of antihypertensive therapy on myocardial fibrosis in hypertensive left ventricular hypertrophy","award":["112102310223"],"award-info":[{"award-number":["112102310223"]}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNA\u2010134\u20105p (MiR\u2010134\u20105p) has been proposed as a promising novel biomarker for the diagnosis of acute myocardial infarction (AMI). However, the biological role of miR\u2010134\u20105p in ischemic cardiomyocytes has been little disclosed yet. Expression of miR\u2010134\u20105p and X\u2010linked inhibitor of apoptosis protein (XIAP) was detected using RT\u2010qPCR and western blot. Oxidative stress and cell apoptosis were determined by enzyme\u2010linked immunosorbent assays, 3\u2010(4, 5\u2010dimethylthiazole\u20102\u2010y1)\u20102, 5\u2010biphenyl tetrazolium bromide assay, flow cytometry, western blot, and terminal\u2010deoxynucleoitidyl transferase\u2010mediated nick end labeling (TUNEL). The interaction between miR\u2010134\u20105p and XIAP was confirmed by luciferase reporter assay. Expression of miR\u2010134\u20105p was upregulated in serum of AMI patients and hypoxia\/reoxygenation (H\/R)\u2010induced cardiomyocytes (AC16 and HCM). MiR\u2010134\u20105p downregulation could inhibit H\/R\u2010mediated release of lactic dehydrogenase enzyme (LDH) and malondialdehyde (MDA), and promote superoxide dismutase (SOD) and glutathione peroxidase (GSH\u2010PX) levels. Meanwhile, cell viability was increased, while the apoptosis rate and TUNEL positive cells were inhibited by miR\u2010134\u20105p downregulation in H\/R\u2010treated AC16 and HCM cells. Mechanically, XIAP was downregulated and targeted by miR\u2010134\u20105p in H\/R\u2010induced cardiomyocytes in vitro. Overexpression of XIAP inhibited oxidative stress and cell apoptosis in H\/R\u2010treated AC16 and HCM cells, which was similar to miR\u2010134\u20105p knockdown. Moreover, downregulation of XIAP could partially reverse the effect of miR\u2010134\u20105p knockdown in H\/R\u2010induced cardiomyocytes. Knockdown of miR\u2010134\u20105p protected cardiomyocytes from H\/R\u2010induced oxidative stress and apoptosis in vitro through targeting XIAP.<\/jats:p>","DOI":"10.1002\/iub.2351","type":"journal-article","created":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T06:32:55Z","timestamp":1597473175000},"page":"2154-2166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["<scp>MiR<\/scp>\u2010134\u20105p targeting <scp>XIAP<\/scp> modulates oxidative stress and apoptosis in cardiomyocytes under hypoxia\/reperfusion\u2010induced injury"],"prefix":"10.1002","volume":"72","author":[{"given":"Min","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Cardiologry Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine  Zhengzhou Henan 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