{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:37:43Z","timestamp":1792805863648,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diabetic nephropathy (DN) is the primary reason of chronic kidney disease. The aim of our study is to explore the role and action mechanism of M2 macrophage\u2010derived exosomes in high glucose (HG)\u2010induced podocytes injury. Here, 30\u2009mmol\/L of HG was used to induce podocytes injury. Annexin V\u2010FITC\/PI double staining was performed to measure podocytes apoptosis, and western blot was carried out to ensure proteins expression. The shape of exosomes was identified using TEM. Besides, the expression of miR\u201025\u20103p was determined by qRT\u2010PCR, FAM\u2010labeled miR\u201025\u20105p combined with DiI\u2010labeled exosomes were utilized to explore the uptake of podocytes to exosomes. Relationship between miR\u201025\u20103p and DUSP family members was ensued by luciferase activity assay. In the beginning, we found that M2 macrophage ameliorated HG\u2010induced podocytes apoptosis and epithelial\u2010mesenchymal transition through secreting exosomes. Subsequently, highly expressed miR\u201025\u20103p was found in M2 macrophage\u2010derived exosomes that effectively improved HG\u2010induced podocytes injury. Furthermore, inhibition of miR\u201025\u20103p in M2 macrophage inefficiently repressed HG\u2010induced podocytes injury, thus we proposed that M2 macrophage attenuated podocytes injury through secreting exosomal miR\u201025\u20103p. Then, we used an autophagy inhibitor to stimulate podocytes, and demonstrated that M2 macrophage\u2010derived exosomal miR\u201025\u20103p improved HG\u2010induced podocytes injury through activating autophagy. Finally, DUSP1 was proved to be a downstream target and mediated the inhibition of exosomal miR\u201025\u20103p to HG\u2010induced podocytes injury. Our results indicated that M2 macrophage could improve HG\u2010induced podocytes injury via secreting exosomal miR\u201025\u20103p to activate autophagy of the cells through suppressing DUSP1 expression. We proved a newly potential therapy strategy for DN treatment.<\/jats:p>","DOI":"10.1002\/iub.2393","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T03:11:42Z","timestamp":1603768302000},"page":"2651-2662","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["<scp>M2<\/scp> macrophage\u2010derived exosomal <scp>miR<\/scp>\u201025\u20103p improves high glucose\u2010induced podocytes injury through activation autophagy via inhibiting <scp>DUSP1<\/scp> expression"],"prefix":"10.1002","volume":"72","author":[{"given":"Haihua","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism The First Affiliated Hospital of Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Fengcheng 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