{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:46:58Z","timestamp":1792176418836,"version":"4.4.0"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Our previous study showed that silencing of lncRNA Gm14461 alleviated pain in a murine model of trigeminal neuralgia (TN), but the molecular mechanism remains not fully understood. Evidence indicates that astrocyte activation and autophagy are involved in the development of TN. Herein, this study aimed to elucidate whether the pain\u2010relief effect of Gm14461 silencing in TN involved regulation of astrocyte activation and autophagy. A murine model of TN was induced by chronic constriction injury of the infraorbital nerve surgery. The mechanical withdrawal threshold (MWT) was measured to assess the analgesic effect of Gm14461 silencing. Mouse astrocytes were treated with lipopolysaccharide (LPS) as a cell model. Astrocyte activation was evaluated by glial fibrillary acidic protein (GFAP) immunofluorescence and western blot analysis of GFAP. Autophagy was evaluated by LC3 immunofluorescence and western blot analysis of autophagy\u2010related proteins. The results showed that Gm14461 silencing increased MWT value in TN model mice. Meanwhile, Gm14461 silencing inhibited astrocyte activation and enhanced autophagy in both TN mice and LPS\u2010treated astrocytes. The enhancement of autophagy by Gm14461 silencing involved the activation of the AMPK signaling and the suppression of the Akt\/mTOR signaling. Collectively, the analgesic effect of Gm14461 silencing in TN was related to attenuation of astrocyte activation via enhancement of autophagy.<\/jats:p>","DOI":"10.1002\/iub.2395","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T18:15:12Z","timestamp":1604427312000},"page":"2663-2671","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Silencing of <scp>lncRNA<\/scp> Gm14461 alleviates pain in trigeminal neuralgia through inhibiting astrocyte activation"],"prefix":"10.1002","volume":"72","author":[{"given":"Jun","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine the First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine the First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengye","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine the First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine the First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuexue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine the First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5081-8842","authenticated-orcid":false,"given":"Mu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine the First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.12998\/wjcc.v7.i9.1060","article-title":"Nerve coblation for treatment of trigeminal neuralgia: A case report","volume":"7","author":"Yang X\u2010H","year":"2019","journal-title":"World J Clin 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