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Parathyroid hormone (PTH) has been identified as a main systemic mediator to balance physiological calcium in bone. This study aimed to uncover PTH's potential role in ameliorating the osteogenic capacity of human bone marrow mesenchymal stem cells (HBMSCs) against glucolipotoxicity. Optimal PTH concentrations and high glucose and palmitic acid (GP) were administered to cells, followed by alkaline phosphatase (ALP) staining and ALP activity assay. Quantitative real\u2010time reverse transcription\u2010polymerase chain reaction (qRT\u2010PCR) and Immunoblot were carried out for assessing mRNA and protein amounts, respectively. Cell counting kit\u20108 (CCK\u20108) and flow cytometry were performed for quantitating cell proliferation. Osteogenesis and oxidative stress were determined, and the involvement of mitogen\u2010activated protein kinase (MAPK) signaling was further verified. About 1\u201350\u2009mmol\/ml GP significantly inhibited the osteogenic differentiation of HBMSCs. 10<jats:sup>\u22129<\/jats:sup> mol\/L PTH was found to be the optimal concentration for HBMSC induction. PTH had no effects on HBMSC proliferation, with or without GP treatment. PTH reversed inadequate osteogenesis and excessive oxidative stress in GP\u2010treated HBMSCs. Mechanistically, PTH activated p38 MAPK signaling, while inhibiting p38 MAPK\u2010suppressed PTH's beneficial impacts on HBMSCs. Collectively, PTH promotes osteogenic differentiation in HBMSCs against glucolipotoxicity via p38 MAPK signaling.<\/jats:p>","DOI":"10.1002\/iub.2420","type":"journal-article","created":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T07:00:40Z","timestamp":1606633240000},"page":"213-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Parathyroid hormone ameliorates osteogenesis of human bone marrow mesenchymal stem cells against glucolipotoxicity through p38 <scp>MAPK<\/scp> signaling"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5500-3351","authenticated-orcid":false,"given":"Yuli","family":"Wang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University  Nanjing China"},{"name":"Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology Nanjing Medical University  Nanjing 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