{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:31:46Z","timestamp":1780345906949,"version":"3.54.1"},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001869","name":"Academia Sinica","doi-asserted-by":"publisher","award":["034007"],"award-info":[{"award-number":["034007"]}],"id":[{"id":"10.13039\/501100001869","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["StG\u2010716734"],"award-info":[{"award-number":["StG\u2010716734"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["CDA00084\/2015\u2010C"],"award-info":[{"award-number":["CDA00084\/2015\u2010C"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Vibrio cholerae<\/jats:italic> is the causative agent of the diarrheal disease cholera, for which biofilm communities are considered to be environmental reservoirs. In endemic regions, and after algal blooms, which may result from phosphate enrichment following agricultural runoff, the bacterium is released from biofilms resulting in seasonal disease outbreaks. However, the molecular mechanism by which <jats:italic>V. cholerae<\/jats:italic> senses its environment and switches lifestyles from the biofilm\u2010bound state to the planktonic state is largely unknown. Here, we report that the major biofilm scaffolding protein RbmA undergoes autocatalytic proteolysis via a phosphate\u2010dependent induced proximity activation mechanism. Furthermore, we show that RbmA mutants that are defective in autoproteolysis cause <jats:italic>V. cholerae<\/jats:italic> biofilms to grow larger and mechanically stronger, correlating well with the observation that RbmA stability directly affects microbial community homeostasis and rheological properties. In conclusion, our biophysical study characterizes a novel phosphate\u2010dependent breakdown pathway of RbmA, while microbiological data suggest a new, sensory role of this biofilm scaffolding element.<\/jats:p>","DOI":"10.1002\/iub.2439","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T02:49:50Z","timestamp":1609210190000},"page":"418-431","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp><i>Vibrio cholerae<\/i><\/scp> biofilm scaffolding protein <scp>RbmA<\/scp> shows an intrinsic, phosphate\u2010dependent autoproteolysis activity"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9566-7216","authenticated-orcid":false,"given":"Manuel","family":"Maestre\u2010Reyna","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"},{"name":"RIKEN SPring\u20108 Center  Sayo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jin","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Terrestrial Microbiology  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimo","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Terrestrial Microbiology  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7359-934X","authenticated-orcid":false,"given":"Po\u2010Hsun","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Chung","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Hikima","sequence":"additional","affiliation":[{"name":"RIKEN SPring\u20108 Center  Sayo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"RIKEN SPring\u20108 Center  Sayo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Bessho","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"},{"name":"RIKEN SPring\u20108 Center  Sayo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Knut","family":"Drescher","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Terrestrial Microbiology  Marburg Germany"},{"name":"Department of Physics Philipps University Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Daw","family":"Tsai","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0016-5337","authenticated-orcid":false,"given":"Andrew H.\u2010J.","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry Academia Sinica  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003832"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb19852.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01569-06"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1601702113"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222981"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04797.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002210"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00374-13"},{"key":"e_1_2_10_10_1","first-page":"8","article-title":"Structural insights into RbmA, a biofilm scaffolding protein of V. cholerae","volume":"82458","author":"Maestre\u2010Reyna M","year":"2013","journal-title":"PLoS ONE"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41567-018-0356-9"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512424112"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.26163"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3433"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.09.041"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.12.019"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00048-10"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2005.00863.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hal.2008.08.006"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1998.tb05279.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00388803"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.12"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.11-0181"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-1688.2010.00448.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.10964"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3065"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201100569"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047337"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911001314"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889805038987"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012458999"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889812007662"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807002853"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1051\/anphys\/193911120161"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892001663"},{"key":"e_1_2_10_38_1","unstructured":"DelanoW.The PyMOL molecular graphics system;2008. Version 2.3.4 Schr\u00f6dinger LLC. Note that this is the citation provided by Schroedinger LLC:https:\/\/pymol.org\/2\/support.html"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw389"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201405664"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00098-16"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.10.030"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00793-8"},{"key":"e_1_2_10_44_1","first-page":"735423","article-title":"BiofilmQ, a software tool for quantitative image analysis of microbial biofilm communities","author":"Hartmann R","year":"2019","journal-title":"bioRxiv"},{"key":"e_1_2_10_45_1","first-page":"151","volume-title":"Chemical kinetics and dynamics","author":"Steinfeld JI","year":"1999"},{"key":"e_1_2_10_46_1","unstructured":"BergJM TymoczkoJL StryerL.The Michaelis\u2010Menten Model Accounts for the Kinetic Properties of Many Enzymes. in Biochemistry 5th Edition 139\u2013146 (W H Freeman 2002)."},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00706-019-02437-z"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1088\/0034-4885\/66\/10\/R05"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2015.03.021"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.112359"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31935-z"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2016.03.003"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004228"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1983.tb18116.x"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920312804871166"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509359403"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/35017531"},{"key":"e_1_2_10_58_1","volume-title":"Nutrients in the nation's eaters\u2014Too much of a good thing?","author":"Mueller DK","year":"1999"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-2647(93)90050-M"},{"key":"e_1_2_10_60_1","first-page":"24","volume-title":"Elsevier Oceanography Series","author":"Mero JL","year":"1965"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(99)00065-5"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.82.4.455"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.9.5079-5088.2003"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2439","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iub.2439","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T13:09:06Z","timestamp":1693746546000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.2439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,28]]},"references-count":62,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/iub.2439"],"URL":"https:\/\/doi.org\/10.1002\/iub.2439","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,28]]},"assertion":[{"value":"2020-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}