{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:02:12Z","timestamp":1772996532326,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,1,31]],"date-time":"2021-01-31T00:00:00Z","timestamp":1612051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Postmenopausal osteoporosis (PMOP) is mainly caused by multiple factors. Recent studies have suggested that iron accumulation (IA) was closely related to PMOP. However, the detailed molecular mechanisms have not been well demonstrated. We constructed the IA mouse model by intraperitoneal injections of ferric ammonium citrate (FAC) and cell model by culturing with the medium containing FAC. Osteoporosis was confirmed in mouse bone tissues using H&amp;E staining, and the level of serum ferritin, alkaline phosphatase (ALP), procollagen\u20101 N\u2010terminal peptide (P1NP), and osteocalcin in mice was examined by ELISA. The expressions of XIST and miR\u2010758\u20103p were detected by qRT\u2010PCR. Cell proliferation and apoptosis were measured by CCK\u20108, TUNEL, and flow cytometry. The expression levels of apoptotic\u2010related proteins were evaluated by western blot. Dual luciferase reporter assay was used to examine the molecular interaction. The expressions of ALP, P1NP, and osteocalcin, and the H&amp;E staining of bone tissues in mice were analyzed to confirm the biological function of XIST and miR\u2010758\u20103p in vivo. XIST was up\u2010regulated while miR\u2010758\u20103p was down\u2010regulated in IA mouse and cell models. XIST knockdown significantly reduced FAC\u2010induced osteoblast apoptosis, which was mimicked by transfection with miR\u2010758\u20103p mimics. XIST acted as a sponge of miR\u2010758\u20103p, which targeted caspase 3. IA led to the high expression of XIST and promoted osteoblast apoptosis through miR\u2010758\u20103p\/caspase 3. Transfection with shXIST or miR\u2010758\u20103p mimics alleviated IA\u2010induced mouse osteoporosis. IA regulated osteoblast apoptosis through XIST\/miR\u2010758\u20103p\/caspase 3 axis, which might provide alternative targets for the treatment of osteoporosis.<\/jats:p>","DOI":"10.1002\/iub.2440","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T17:00:08Z","timestamp":1608310808000},"page":"432-443","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Iron accumulation regulates osteoblast apoptosis through <scp>lncRNA<\/scp><scp>XIST<\/scp>\/<scp>miR<\/scp>\u2010758\u20103p\/caspase 3 axis leading to osteoporosis"],"prefix":"10.1002","volume":"73","author":[{"given":"Hu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Soochow University  Suzhou China"},{"name":"Department of Orthopedics Yancheng Third People's Hospital  Yancheng 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