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Clearly, induction of apoptosis in seminiferous tubules leads to abnormalities in spermatogenesis and male infertility. Studies demonstrated that increased hyperlipidemia impairs male infertility and spermatogenesis by enhancing seminiferous tubules apoptosis. However, molecular mechanisms underlying high\u2010cholesterol\u2010mediated testicular damage remain poorly elucidated. In this scope, we established a rabbit model and investigated the role of endoplasmic reticulum (ER) stress on high cholesterol diet induced seminiferous tubule apoptosis. Histopatological examinations revealed increased seminifer tubule apoptosis in testes of rabbits fed high cholesterol diet. In addition, phosphorylated forms of IRE1 and PERK, two well\u2010identified markers of ER stress, were significantly induced in accordance with high cholesterol diet. High cholesterol diet also exhibited CHOP induction in testes, indicating increased ER stress related apoptosis. Supplementation of \u03b1\u2010tocopherol significantly attenuated cholesterol mediated ER stress, and restored seminiferous tubules apoptosis. Taken together, our findings suggest that \u03b1\u2010tocopherol might be capable to reduce testicular damage via ameliorating histopatological features and inhibiting seminiferous tubules apoptosis in hypercholesterolemic rabbits.<\/jats:p>","DOI":"10.1002\/iub.2535","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T06:00:52Z","timestamp":1628143252000},"page":"85-92","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["High cholesterol diet activates <scp>ER<\/scp> stress mediated apoptosis in testes tissue: Role of <scp>\u03b1\u2010tocopherol<\/scp>"],"prefix":"10.1002","volume":"74","author":[{"given":"Erdi","family":"Sozen","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Biochemistry Marmara University  Istanbul Turkey"},{"name":"Genetic and Metabolic Diseases Research and Investigation Center (GEMHAM) Marmara University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tugce","family":"Demirel\u2010Yalciner","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Biochemistry Marmara University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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