{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:34:42Z","timestamp":1773300882775,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Posttranslational modifications (PTMs) could influence many aspects of protein behavior and function in organisms. Protein glycosylation is one of the major PTMs observed in bacteria, which is crucial for functional regulations of many prokaryotic and eukaryotic organisms. <jats:italic>Mycobacterium bovis<\/jats:italic> bacillus Calmette\u2013Gu\u00e9rin (BCG) vaccine has been recognized as an indispensable tool in the global fight against tuberculosis (TB) worldwide over several decades. Nevertheless, analysis of glycoprotein profiles of BCG has not been clearly investigated. In this study, we performed <jats:italic>O<\/jats:italic>\u2010mannosylated protein analysis in BCG bacteria using gel\u2010based and gel\u2010free approaches. In total, 1,670 hexosylated peptides derived from 754 mannosylated proteins were identified. Furthermore, 20 novel protein products supported by 78 unique peptides not annotated in the BCG database were detected. Additionally, the translational start sites of 384 proteins were confirmed, and 78 proteins were validated through the extension of translational start sites based on N\u2010terminus\u2010derived peptides. The bioinformatic analysis of the <jats:italic>O<\/jats:italic>\u2010mannosylated proteins was performed and the expression profiles of four randomly selected proteins were validated through Western blotting. A number of proteins involved in metabolic pathways, including the tricarboxylic acid cycle, glycolysis, oxidative phosphorylation, and two\u2010component system, are discussed. Taken together, these results offer the first <jats:italic>O<\/jats:italic>\u2010mannosylated protein analysis of a member of mycobacteria reported to date by using complementary gel\u2010based and gel\u2010free approaches. Some of the proteins identified in this study have important roles involved in metabolic pathways, which could provide insight into the immune molecular mechanisms of this recognized vaccine strain.<\/jats:p>","DOI":"10.1002\/iub.2578","type":"journal-article","created":{"date-parts":[[2021,11,13]],"date-time":"2021-11-13T13:15:28Z","timestamp":1636809328000},"page":"221-234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of <i>O<\/i>\u2010mannosylated proteins profiling in bacillus <scp>Calmette\u2013Gu\u00e9rin<\/scp> via gel\u2010based and gel\u2010free approaches"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6000-0326","authenticated-orcid":false,"given":"Jianhua","family":"Zheng","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liguo","family":"Liu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Candong","family":"Wei","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Jin","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, and Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68384-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2021.02.107"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2020.12.018"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1211"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12968"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbab089"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwy093"},{"key":"e_1_2_9_9_1","article-title":"Recent advancements in glycoproteomic studies: Glycopeptide enrichment and derivatization, characterization of glycosylation in SARS CoV2, and interacting glycoproteins","author":"Pujic I","year":"2021","journal-title":"Mass Spectrom Rev"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00620-15"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900937w"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"M000031\u2010MCP201","DOI":"10.1074\/mcp.M000031-MCP201","article-title":"Simultaneous glycan\u2010peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N\u2010linked glycoproteome of Campylobacter jejuni","volume":"10","author":"Scott NE","year":"2011","journal-title":"Mol Cell Proteom"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.029561"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/fty082"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/b916394h"},{"key":"e_1_2_9_16_1","first-page":"361","article-title":"Toward understanding the essence of post\u2010translational modifications for the Mycobacterium tuberculosis immunoproteome","volume":"5","author":"Els CA","year":"2014","journal-title":"Front Immunol"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr800756a"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.05.011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0221837"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.09.010"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700342"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.057505"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.07.017"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.027318"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac103243t"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01816"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900001t"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-777"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818005.ch12"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.14.2227213"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.028878-0"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8479(96)90120-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi011518y"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01472-09"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12575"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003116"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.01.039"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0311095"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/213137a0"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.66.1.717"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg608"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(01)00238-9"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-242"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06258-11"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.060319-0"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.8.2192-2203.2002"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1998.0265"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00240"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2016.05.006"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0133186"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.671164"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-010-0583-7"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8378-6"},{"key":"e_1_2_9_54_1","first-page":"664","article-title":"Mycobacterium bovis Mce4E protein may play a role in modulating cytokine expression profile in alveolar macrophage","volume":"12","author":"Xu G","year":"2008","journal-title":"Int J Tuberc Lung Dis"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-60"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4285"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.05.034"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/001537-0"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth390"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2578","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iub.2578","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T12:50:58Z","timestamp":1693140658000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.2578"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/iub.2578"],"URL":"https:\/\/doi.org\/10.1002\/iub.2578","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]},"assertion":[{"value":"2021-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}