{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:35:27Z","timestamp":1787859327079,"version":"build-2784847793"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,2,20]],"date-time":"2022-02-20T00:00:00Z","timestamp":1645315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500577"],"award-info":[{"award-number":["81500577"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bladder outlet obstruction (BOO) is a type of chronic disease that is mainly caused by benign prostatic hyperplasia. Previous studies discovered the involvements of both serum\/glucocorticoid\u2010regulated kinase 1 (SGK1) and activated T cell nuclear factor transcription factor 2 (NFAT2) in the proliferation of smooth muscle cells after BOO. However, the relationship between these two molecules is yet to be explored. Thus, this study explored the specific mechanism of the SGK1\u2010NFAT2 signaling pathway in mouse BOO\u2010mediated bladder smooth muscle cell proliferation in vivo and in vitro. In vivo experiments were performed by suturing 1\/2 of the external urethra of female BALB\/C mice to cause BOO for 2\u2009weeks. In vitro, mouse bladder smooth muscle cells (MBSMCs) were treated with dexamethasone (Dex) or dexamethasone\u2009+\u2009SB705498 for 12\u2009h and were transfected with SGK1 siRNA for 48\u2009h. The expression and distribution of SGK1, transient receptor potential oxalate subtype 1 (TRPV1), NFAT2, and proliferating cell nuclear antigen (PCNA) were measured by Western blotting, polymerase chain reaction, and immunohistochemistry. The relationship between SGK1 and TRPV1 was analyzed by <jats:bold>coimmunoprecipitation<\/jats:bold>. The proliferation of MBSMCs was examined by 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine and cell counting kit 8 assays. Bladder weight, smooth muscle thickness, and collagen deposition in mice after 2\u2009weeks of BOO were examined. Bladder weight, smooth muscle thickness, the collagen deposition ratio, and the expression of SGK1, TRPV1, NFAT2, and PCNA were significantly increased in mice after 2\u2009weeks of BOO. Compared with the control, 10\u2009\u03bcM Dex promoted the expression of these four molecules and the proliferation of MBSMCs. After inhibiting TRPV1, only the expression of SGK1 was not affected, and the proliferation of MBSMCs was inhibited. After silencing SGK1, the expression of these four molecules and the proliferation of MBSMCs decreased. Coimmunoprecipitation suggested that SGK1 acted directly on TRPV1. In this study, SGK1 targeted TRPV1 to regulate the proliferation of MBSMCs mediated by BOO in mice through NFAT2 and then affected the process of bladder remodeling after BOO. This finding may provide a strategy for BOO drug target screening.<\/jats:p>","DOI":"10.1002\/iub.2605","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T18:13:00Z","timestamp":1644603180000},"page":"463-473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serum\/glucocorticoid\u2010regulated kinase 1\u2010targeted transient receptor potential oxalate subtype 1 regulates bladder smooth muscle cell proliferation due to bladder outlet obstruction in mice via activated T cell nuclear factor transcription factor 2"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0399-8585","authenticated-orcid":false,"given":"Jiangshu","family":"He","sequence":"first","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"},{"name":"Department of Urology Zunyi Medical University  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"},{"name":"Department of Urology Zunyi Medical University  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"},{"name":"Department of Urology Zunyi Medical University  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinglin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7279-0895","authenticated-orcid":false,"given":"Xun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Xichang People's Hospital  Xichang Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taotao","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"},{"name":"Department of Urology Zunyi Medical University  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"},{"name":"Department of Urology Zunyi Medical University  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Hospital of Chengdu University  Chengdu Sichuan China"},{"name":"Department of Urology Zunyi Medical University  Zunyi Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amend","family":"Bastian","sequence":"additional","affiliation":[{"name":"Department of Urology University of T\u00fcbingen  T\u00fcbingen Baden\u2010W\u00fcrttemberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stenzl","family":"Arnulf","sequence":"additional","affiliation":[{"name":"Department of Urology University of T\u00fcbingen  T\u00fcbingen Baden\u2010W\u00fcrttemberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2018.02.009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2014.03.037"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107947"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-0357-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.15698\/cst2019.01.170"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019001866"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61667-y"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2017.11.010"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2019.104290"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.03.112"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11092"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01064"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina55070380"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2019.04.015"},{"key":"e_1_2_10_17_1","first-page":"55","article-title":"Is it time for an expanded role of dexmedetomidine in contemporary anesthesia practice? A clinician's perspective","volume":"5","author":"Bohringer C","year":"2018","journal-title":"Transl Perioper Pain Med"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21427"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.59918"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00064.2017"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18136-w"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-013-9938-3"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6712536"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-018-0329-4"},{"key":"e_1_2_10_25_1","first-page":"2088","article-title":"Suture causing urethral meatus stricture: A novel animal model of partial bladder outlet obstruction","volume":"37","author":"Chen L","year":"2018","journal-title":"NeurourolUrodyn"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/stke.2001.108.re17"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.3451"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-021-02942-3"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.10.117"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2605","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iub.2605","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.2605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T19:56:06Z","timestamp":1692993366000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.2605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,20]]},"references-count":28,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1002\/iub.2605"],"URL":"https:\/\/doi.org\/10.1002\/iub.2605","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,20]]},"assertion":[{"value":"2021-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}