{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:12:52Z","timestamp":1792271572872,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009592","name":"Beijing Municipal Science and Technology Commission","doi-asserted-by":"publisher","award":["7212174"],"award-info":[{"award-number":["7212174"]}],"id":[{"id":"10.13039\/501100009592","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82004045"],"award-info":[{"award-number":["82004045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Acetaminophen (APAP), one of the most widely used antipyretics and analgesics, principally results in acute liver injury (ALI) in developed countries when taken overdose. Ferulic acid (FA) is a natural polyphenol compound existing in many plants that has free radical scavenging, anti\u2010inflammatory, and liver\u2010protective properties. However, the effect and underlying mechanism of FA in treating APAP\u2010induced ALI have not been fully elucidated. Herein, we established a mouse model of APAP\u2010induced ALI and used APAP\u2010stimulated MPHs for biochemical assessment of molecular parameters. After constructing networks and obtaining predicted targets from public databases, we further verified the putative pathways using immune\u2010blotting assays both in vivo and in vitro. The reign of liver necrosis, serum levels of ALT and AST, and oxidative stress in livers significantly elevated after APAP treatment, which were almost recovered back to normal levels by FA administration. In addition, FA significantly upregulated the APAP\u2010induced downregulation of hepatic specific markers, including\n                    <jats:italic>HNF4a<\/jats:italic>\n                    ,\n                    <jats:italic>Foxa2<\/jats:italic>\n                    , and ALB. Then, the results of functional enrichment indicated the possible signaling pathways of FA against APAP challenge, mainly including AMPK, autophagy, apoptosis, and other metabolic process. Furthermore, FA markedly reversed the APAP\u2010induced decline of mitochondria membrane potential, increased ratio of BAX\/BCL2 and CASPASE 3 expression, and promoted autophagy flux of hepatocytes by upregulating AMPK phosphorylation, which were abrogated by a specific AMPK inhibitor, compound C. Overall, the hepatoprotective effect of FA on APAP\u2010induced ALI might be associated with anti\u2010oxidant and anti\u2010apoptosis, which were at least partly attributed to AMPK\u2010mediated protective autophagy.\n                  <\/jats:p>","DOI":"10.1002\/iub.2625","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T01:14:40Z","timestamp":1651799680000},"page":"880-895","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Ferulic acid ameliorates acetaminophen\u2010induced acute liver injury by promoting\n                    <scp>AMPK<\/scp>\n                    \u2010mediated protective autophagy"],"prefix":"10.1002","volume":"74","author":[{"given":"Jianzhi","family":"Wu","sequence":"first","affiliation":[{"name":"School of Life Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifang","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Beijing University of Chinese Medicine  Beijing 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