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Altered iron metabolism is a feature of cancer cells. Using EM, we examined the plausible relationship between microtubule depolymerization and induction of ferroptosis in human neuroblastoma (SH\u2010SY5Y and IMR\u201032) cells. EM reduced glutathione (GSH) levels and induced reactive oxygen species (ROS) generation. The pre\u2010treatment of neuroblastoma cells with ROS scavengers (N\u2010acetyl cysteine and dithiothreitol) reduced the anti\u2010proliferative effects of EM. EM treatment increased labile iron pool (LIP), depleted glutathione peroxidase 4 (GPX4) levels, and lipid peroxidation, hallmark features of ferroptosis, highlighting ferroptosis induction. Ferroptosis inhibitors (deferoxamine mesylate and liproxstatin\u20101) abrogated the cytotoxic effects of EM, further confirming ferroptosis induction. Vinblastine and nocodazole also increased LIP and induced lipid peroxidation in neuroblastoma cells. This study provides evidence for the coupling of microtubule integrity to ferroptosis. The results also suggest that microtubule\u2010depolymerizing agents may be considered for developing pro\u2010ferroptosis chemotherapeutics.<\/jats:p>","DOI":"10.1002\/iub.2899","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T17:40:27Z","timestamp":1721670027000},"page":"1186-1198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Microtubule depolymerization induces ferroptosis in neuroblastoma cells"],"prefix":"10.1002","volume":"76","author":[{"given":"Mayuri","family":"Bandekar","sequence":"first","affiliation":[{"name":"Department of Biosciences &amp; Bioengineering Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9885-4500","authenticated-orcid":false,"given":"Dulal","family":"Panda","sequence":"additional","affiliation":[{"name":"Department of Biosciences &amp; 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