{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T18:22:29Z","timestamp":1750789349476},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,7]],"date-time":"2010-04-07T00:00:00Z","timestamp":1270598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bladder carcinoma is an important worldwide health problem. Both cystoscopy and urine cytology used in detecting bladder cancer suffer from drawbacks where cystoscopy is an invasive method and urine cytology shows low sensitivity in low\u2010grade tumors. This study validates easier and less time\u2010consuming techniques for the estimation of survivin and TIMP\u20102 in urine of bladder cancer patients to evaluate them in comparison with cytology. This study includes malignant (bladder cancer patients, <jats:italic>n<\/jats:italic> = 42), benign (patients with bilharzial cystitis, <jats:italic>n<\/jats:italic> = 22) and healthy (<jats:italic>n<\/jats:italic> = 21) groups. The studied groups were subjected to cystoscopic examination, detection of bilharzial antibodies, urine cytology, and estimation of urinary survivin by qualitative RT\u2010nested PCR and TIMP\u20102 by ELISA. Significantly higher positivity rates of urinary survivin and TIMP\u20102 were observed in the malignant group compared with benign and healthy groups. On associating the two urinary markers with different clinicopathological factors, only TIMP\u20102 exerted significantly higher positivity rate in invasive stage (100%) than superficial stage (82.3%). Survivin showed 78.6% sensitivity, 95.3% specificity, 94.3% PPV, 82% NPV, and 87% accuracy. When combined with urine cytology, the sensitivity increased to 83.3%. While on applying the cutoff value of urinary TIMP\u20102 (\u2264639.5 pg\/mg protein), it showed 93% sensitivity, 83.7% specificity, 85% PPV, 92.3% NPV, and 88.2% accuracy. When combined with urine cytology, the TIMP\u20102 sensitivity remained 93%. On combining cytology with both urinary survivin and TIMP\u20102, the highest sensitivity was reached (98%). Survivin and TIMP\u20102 can be considered as potentially useful urine markers in early detection of bladder cancer. \u00a9 2010 IUBMB IUBMB Life, 62(5): 394\u2013399, 2010<\/jats:p>","DOI":"10.1002\/iub.325","type":"journal-article","created":{"date-parts":[[2010,4,7]],"date-time":"2010-04-07T19:25:04Z","timestamp":1270668304000},"page":"394-399","source":"Crossref","is-referenced-by-count":20,"title":["Diagnostic evaluation of apoptosis inhibitory gene and tissue inhibitor matrix metalloproteinase\u20102 in patients with bladder cancer"],"prefix":"10.1002","volume":"62","author":[{"given":"Sanaa","family":"Eissa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marwa I.","family":"Shabayek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal F.","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raouf M.","family":"El\u2010Allawy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Hamdy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-007-0223-2"},{"key":"e_1_2_5_3_2","first-page":"127","article-title":"Systemic management of bladder cancer in Egypt","volume":"17","author":"Khaled H. 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