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However, 30%\u201340% of MAFLD cases progress to metabolic dysfunction\u2010associated steatohepatitis (MASH), increasing the importance of the disease. MicroRNAs (miRNAs), non\u2010coding RNA molecules approximately 21 nucleotides long, are used as biomarkers in many diseases and play a crucial role in regulating cellular processes by affecting gene expression. It is also known that miRNAs are effective in the progression of MASH and its profile depends on the stage of the disease. Therefore, we determined the relationship between MASH and miRNA profiles in an in\u00a0vivo trial using an established model of cholesterol\u2010induced MASH in rabbits. We also evaluated the impact of \u03b1\u2010tocopherol, which is known to have a protective effect in MAFLD\/MASH transition, on miRNA profiles. Regarding the limited information using rabbits, we first performed miRNA screening and identified miRNAs that are already described in rabbits or other organisms as well as the putative ones. Among those, two putative miRNAs (miR\u2010230 and miR\u20101146) determined by sequencing may be important in the diagnosis and treatment of the disease. Furthermore, levels of five miRNAs (miR\u2010122\u20105p, miR\u2010199\u20105p, miR\u2010145\u20105p, miR\u201027b\u20103p, miR\u201034a\u20105p) and their relevance in the pathogenesis of MASH were determined by RT\u2010PCR and target gene prediction, respectively. In conclusion, the present study provides novel information regarding dysregulated miRNAs in high\u2010cholesterol diet\u2010induced MASH and the impact of \u03b1\u2010tocopherol.<\/jats:p>","DOI":"10.1002\/iub.70063","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T10:29:45Z","timestamp":1758623385000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of <scp>miRNA<\/scp> Expression Profiles in High Cholesterol Diet\u2010Induced <scp>MASH<\/scp> Progression: Potential Effect of \u03b1\u2010Tocopherol"],"prefix":"10.1002","volume":"77","author":[{"given":"Tugce","family":"Demirel\u2010Yalciner","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Uskudar University  Istanbul Turkey"},{"name":"Metabolic and Inflammatory Diseases Research Center (METIFLAM) Uskudar University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengu","family":"Cetinkaya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Marmara University  Istanbul Turkey"},{"name":"Institute of Health Sciences Marmara University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazim Yalcin","family":"Arga","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Faculty of Engineering Marmara University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6046-7679","authenticated-orcid":false,"given":"Erdi","family":"Sozen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Marmara University  Istanbul Turkey"},{"name":"Genetic and Metabolic Diseases Research Center (GEMHAM) Marmara University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4968-3178","authenticated-orcid":false,"given":"Nesrin Kartal","family":"Ozer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Uskudar University  Istanbul Turkey"},{"name":"Metabolic and Inflammatory Diseases Research Center (METIFLAM) Uskudar University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32511-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-023-01072-3"},{"key":"e_1_2_8_4_1","first-page":"1617","article-title":"MicroRNAs: Biomarkers, Diagnostics, and Therapeutics","volume":"3","author":"Huang W.","year":"2017","journal-title":"Bioinformatics in MicroRNA Research"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.107615"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24081509"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-320"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28854-4"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/03000605231197058"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2022.101581"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11050989"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4137\/GRSB.S6007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1700"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.08.024"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010079"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.09.032"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.03.353"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(96)05909-6"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2014.02.017"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8520746"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26080-6"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-5-1-r1"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btae615"},{"issue":"1","key":"e_1_2_8_25_1","article-title":"The Gene Ontology Knowledgebase in 2023","volume":"224","author":"Aleksander S. 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