{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T03:23:01Z","timestamp":1768792981274,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,7,10]],"date-time":"2008-07-10T00:00:00Z","timestamp":1215648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study evaluates sequence conservation in the gene coding for nitrite reductase (<jats:italic>ani<\/jats:italic>A) and AniA expression from a panel of <jats:italic>Neisseria meningitidis<\/jats:italic> isolates. Sequence analysis of the coding region in 19 disease\u2010associated and 4 carrier strains notwithstanding a high degree of sequence similarity showed a number of nucleotide changes, some of which possibly resulted in premature translation termination or function loss. In particular, in one disease\u2010associated strain a 9\u2010residues insertion was found to be located close to the type I Cu\u2010site and a catalytic histidine at position 280 was mutated into a leucine. In two strains from carriers, a sequence corresponding to a portion of a transposase gene within the <jats:italic>aniA<\/jats:italic> was also found. The AniA protein was always expressed, except for these two carriers strains and for other two strains in which the presence of the premature stop codons was recognized. The biochemical properties of the cloned soluble domain of the enzyme (sAniA) from <jats:italic>N. meningitidis<\/jats:italic> reference MC58 strain and from a clinical invasive isolate were studied. In particular, biochemical analysis of sAniA from MC58 demonstrated a clear dependence of its catalytic activity upon acidification, while the clinical isolate\u2010derived sAniA was not functional. Thus, the results obtained suggest that the presence of a conserved and functional <jats:italic>ani<\/jats:italic>A gene is not essential for meningococcal survival. \u00a9 2008 IUBMB IUBMB Life, 60(9): 629\u2013636, 2008<\/jats:p>","DOI":"10.1002\/iub.95","type":"journal-article","created":{"date-parts":[[2008,7,10]],"date-time":"2008-07-10T19:42:16Z","timestamp":1215718936000},"page":"629-636","source":"Crossref","is-referenced-by-count":25,"title":["Molecular characterization of nitrite reductase gene (<i>ani<\/i>A) and gene product in <i>Neisseria meningitidis<\/i> isolates: Is <i>ani<\/i>A essential for meningococcal survival?"],"prefix":"10.1002","volume":"60","author":[{"given":"Paola","family":"Stefanelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianni","family":"Colotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arianna","family":"Neri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Luisa","family":"Salucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Miccoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana","family":"Di Leandro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo","family":"Ippoliti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,7,10]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00356-7"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.19888"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.11.2987-2993.2002"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.04.027"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm954"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20664"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-02-0567"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2466"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004380050597"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.5251"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(88)90038-1"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04807.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5459.1809"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0340139"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04866.x"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cber.187901201117"},{"key":"e_1_2_7_19_2","first-page":"934","article-title":"Evaluation of the Griess nitrite test as a method for the recognition of urinary tract infection","volume":"49","author":"Kahler R. 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