{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:19:11Z","timestamp":1779887951323,"version":"3.53.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,6,11]],"date-time":"2012-06-11T00:00:00Z","timestamp":1339372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Pain"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Some studies have shown a somatic nociceptive response due to the activation of transient receptor potential <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>1 channels (<jats:styled-content style=\"fixed-case\">TRPA<\/jats:styled-content>1), which is modulated by the <jats:styled-content style=\"fixed-case\">TRPA<\/jats:styled-content>1 antagonist <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>\u2010030031. However, a few studies report the role of <jats:styled-content style=\"fixed-case\">TRPA<\/jats:styled-content>1 in visceral pain. Therefore, we investigated the participation of <jats:styled-content style=\"fixed-case\">TRPA<\/jats:styled-content>1 in visceral nociception and the involvement of nitric oxide, the opioid system and resident cells in the modulation of these channels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were treated with vehicle or <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>\u2010030031 (18.75\u2013300\u2009mg\/kg) before ifosfamide (400\u2009mg\/kg), 0.75% mustard oil (50\u2009<jats:styled-content style=\"fixed-case\">\u03bcL<\/jats:styled-content>\/colon), acetic acid 0.6% (10\u2009<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>\/kg), zymosan (1\u2009mg\/cavity) or misoprostol (1\u2009\u03bcg\/cavity) injection. Visceral nociception was assessed through the electronic von <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>rey test or the writhing response. Ifosfamide\u2010administered mice were euthanized for bladder analysis. The involvement of nitric oxide and the opioid system were investigated in mice injected with ifosfamide and mustard oil, respectively. The participation of resident peritoneal cells in acetic acid\u2010, zymosan\u2010 or misoprostol\u2010induced nociception was also evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>\u2010030031 failed to protect animals against ifosfamide\u2010induced bladder injury (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). However, a marked antinociceptive effect against ifosfamide, mustard oil, acetic acid, zymosan and misoprostol was observed (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Neither <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\u2010arginine (600\u2009mg\/kg) nor naloxone (2\u2009mg\/kg) could reverse the antinociceptive effect of <jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>\u2010030031. The reduction of the peritoneal cell population inhibited the acetic acid and zymosan\u2010related writhes without interfering with the misoprostol effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that the blockade of <jats:styled-content style=\"fixed-case\">TRPA<\/jats:styled-content>1 attenuates visceral nociception by a mechanism independent of the modulation of resident cells, nitric oxide and opioid pathways.<\/jats:p><\/jats:sec>","DOI":"10.1002\/j.1532-2149.2012.00177.x","type":"journal-article","created":{"date-parts":[[2012,6,11]],"date-time":"2012-06-11T15:55:16Z","timestamp":1339430116000},"page":"223-233","source":"Crossref","is-referenced-by-count":31,"title":["<scp>B<\/scp>lockade of <scp>TRPA<\/scp>1 with <scp>HC<\/scp>\u2010030031 attenuates visceral nociception by a mechanism independent of inflammatory resident cells, nitric oxide and the opioid 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