{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T08:51:13Z","timestamp":1766047873072,"version":"3.32.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,8,30]],"date-time":"2004-08-30T00:00:00Z","timestamp":1093824000000},"content-version":"vor","delay-in-days":3195,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Biomaterials"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heparin oligosaccharides with different anticoagulant activities were prepared and immobilized onto pyrolytic carbon coated graphite (PC) heart valve materials commonly used in mechanical heart valve prostheses. Prior to immobilization, PC surfaces were modified by radio\u2010frequency plasma polymerized<jats:italic>N<\/jats:italic>\u2010vinyl\u20102\u2010pyrrolidone (PPNVP) thin films (\u223c 100 nm) and derivatized to provide surface hydroxyl groups. Cleaved, low affinity heparin (C\u2010heparin) with factor Xa inhibition activity of 107 to 130 IU\/mg, was prepared by partial deaminative cleavage of commercial crude heparin, and high\u2010affinity heparin (HA\u2010heparin) with factor Xa inhibition activity of 550 to 1000 IU\/mg was prepared by fractionation of C\u2010heparin using agarose\u2010ATIII affinity chromatography. C\u2010heparin and HA\u2010heparin were immobilized to surface modified PC by reductive amination. Anticoagulant activity of the heparin immobilized surfaces was determined by chromogenic assay for the inhibition of factor Xa. Highest surface anticoagulant activity was measured on C\u2010heparin immobilized surfaces (64.0 \u00b1 7.3 mIU\/cm<jats:sup>2<\/jats:sup>) compared with HA\u2010heparin immobilized surfaces (27.2 \u00b1 12.2 mIU\/cm<jats:sup>2<\/jats:sup>), suggesting higher binding of C\u2010heparin than HA\u2010heparin on the modified PC surfaces. Immobilized surfaces were evaluated under dynamic flow conditions, by subjecting samples to shear stress of up to 206 dyn\/cm<jats:sup>2<\/jats:sup>in the presence of 5% albumin solution or human plasma. Anticoagulant activity of the immobilized heparin was retained, although reduced, and the modified surfaces showed evidence for protein resistance. \u00a9 1995 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/jab.770060407","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T12:19:17Z","timestamp":1117369157000},"page":"259-266","source":"Crossref","is-referenced-by-count":11,"title":["Immobilization of heparin oligosaccharides onto radiofrequency plasma modified pyrolytic carbon\u2010coated graphite"],"prefix":"10.1002","volume":"6","author":[{"given":"Shengmei","family":"Yuan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weizhen","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyongyi","family":"Szakalas\u2010Gratzl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kandice","family":"Kottke\u2010Marchant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Tweden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger E.","family":"Marchant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,8,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(77)90247-1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1730885"},{"key":"e_1_2_1_4_2","first-page":"235","volume-title":"Biocompatibility: Interactions of biological and implantable materials","author":"Silver F.","year":"1989"},{"issue":"1","key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/j.1097-4636.1987.tb00006.x","article-title":"Cardiac valve prostheses: A review of clinical status and contemporary biomaterials issues","volume":"21","author":"Schoen F. J.","year":"1987","journal-title":"J. Biomed. Mater. Res. Appl. Biomater."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109700"},{"key":"e_1_2_1_7_2","first-page":"717","article-title":"Bioprosthetic heart valve failure: Pathology and pathogenesis Cardiol","volume":"2","author":"Schoen F. J.","year":"1984","journal-title":"Clin."},{"key":"e_1_2_1_8_2","first-page":"103","volume-title":"Chemistry and physics of carbon","author":"Bokros J. C.","year":"1973"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4471-1847-3"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820270614"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/pola.1989.080270314"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1163\/156856292X00123"},{"key":"e_1_2_1_13_2","first-page":"77","article-title":"Surface modification of polyethylene film by plasma polymerization and chemical derivatization","volume":"54","author":"Yuan S.","year":"1994","journal-title":"J. Appl. Polym. Sci.; Polym. Symp."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ma00197a014"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80905-3"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(62)90095-7"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)81050-2"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(83)88103-8"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.3109\/10731198309118804"},{"key":"e_1_2_1_20_2","first-page":"393","volume-title":"Heparin. Chemical and biological properties, clinical applications","author":"Barrowcliffe T. W.","year":"1989"},{"key":"e_1_2_1_21_2","unstructured":"Yuan S.Preparation of nonthrombogenic polymer surfaces: Immobilization of heparin and dextran oligosaccharides. Case Western Reserve University; Ph. D. Thesis;1994:184\u2013193."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.2.329"},{"key":"e_1_2_1_23_2","first-page":"57","volume-title":"Physiochemical hydrodynamics","author":"Levich V. G.","year":"1962"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-0703-5_18"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.142.3597.1297"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820110210"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820200705"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820231309"},{"issue":"2","key":"e_1_2_1_29_2","first-page":"211","article-title":"Evaluation of blood compatibility of PEO\u2010grafted and heparin\u2010immobilized polyurethanes","volume":"23","author":"Han D. K.","year":"1989","journal-title":"J. Biomed. Mater. Res. Appl. Biomater."},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00456a035"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/pola.1990.080280112"},{"key":"e_1_2_1_32_2","first-page":"597","volume-title":"Heparin: Chemical and biological properties, clinical applications","author":"Larm O.","year":"1989"},{"key":"e_1_2_1_33_2","first-page":"102","article-title":"Reduced thrombogenic characteristics of expanded polytetrafluoroethylene and polyurethane","volume":"95","author":"Esquivel C. O.","year":"1984","journal-title":"Surgery"},{"key":"e_1_2_1_34_2","unstructured":"Szakalas\u2010Gratzl G.;Jacobsen D. W.;Marchant K. K.;Marchant R. E.Intermolecular reaction between cleaved oligosaccharide chains of heparin and their anticoagulant activities.Trans. Am. Chem. Soc.1992."},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1163\/156856294X00509"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(82)90149-9"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820190407"},{"key":"e_1_2_1_38_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4899-0703-5","volume-title":"Polyethylene glycol chemistry: Biotechnical and biomedical applications","author":"Harris J. M.","year":"1992"}],"container-title":["Journal of Applied Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjab.770060407","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jab.770060407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T13:34:38Z","timestamp":1735738478000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jab.770060407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,12]]}},"alternative-id":["10.1002\/jab.770060407"],"URL":"https:\/\/doi.org\/10.1002\/jab.770060407","archive":["Portico"],"relation":{},"ISSN":["1045-4861","1549-9316"],"issn-type":[{"type":"print","value":"1045-4861"},{"type":"electronic","value":"1549-9316"}],"subject":[],"published":{"date-parts":[[1995,12]]}}}