{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:16:01Z","timestamp":1760548561289,"version":"build-2065373602"},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,30]],"date-time":"2005-03-30T00:00:00Z","timestamp":1112140800000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Hershberger assay has been proposed as a candidate screening test method for the detection of androgenic and anti\u2010androgenic chemicals and is being validated presently under the test guideline programme of the Organization for Economic Cooperation and Development (OECD). Rat <jats:italic>\u03b1<\/jats:italic><jats:sub>2u<\/jats:sub>\u2010globulin is male rat\u2010specic protein appearing in their serum and urine, and the protein is known to be induced by androgens. We investigated the usefulness of measuring serum <jats:italic>\u03b1<\/jats:italic><jats:sub>2u<\/jats:sub>\u2010globulin levels as a parameter for the androgenic activity of chemicals tested in the Hershberger assay. The serum <jats:italic>\u03b1<\/jats:italic><jats:sub>2u<\/jats:sub>\u2010globulin level was measured after the administration of testosterone propionate at dosages of 0, 20, 100 or 500 \u00b5g kg<jats:sup>\u22121<\/jats:sup> day<jats:sup>\u22121<\/jats:sup> for ten consecutive days in the castrated male rats. The ventral prostate, balbocavernosus[sol ]levator ani muscles, glans penis and Cowper's gland were collected and weighed. Although all the androgen\u2010responsive organ weights were increased signicantly at dosages of 100 and 500 \u00b5g kg<jats:sup>\u22121<\/jats:sup> day<jats:sup>\u22121<\/jats:sup>, the serum <jats:italic>\u03b1<\/jats:italic><jats:sub>2u<\/jats:sub>\u2010globulin level was increased signicantly only at a dosage of 500 \u00b5g kg<jats:sup>\u22121<\/jats:sup> day<jats:sup>\u22121<\/jats:sup>. These results show that the serum <jats:italic>\u03b1<\/jats:italic><jats:sub>2u<\/jats:sub>\u2010globulin level may be a useful biomarker for detecting androgenic activity caused by test chemicals, but it is less sensitive than the organ weights of androgen\u2010responsive tissues in the Hershberger assay. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.1038","type":"journal-article","created":{"date-parts":[[2005,3,30]],"date-time":"2005-03-30T04:48:13Z","timestamp":1112158093000},"page":"176-178","source":"Crossref","is-referenced-by-count":3,"title":["Changes in serum <i>\u03b1<\/i><sub>2u<\/sub>\u2010globulin levels in castrated male rats treated with testosterone propionate in a Hershberger assay"],"prefix":"10.1002","volume":"25","author":[{"given":"Masahiro","family":"Takeyoshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Mitoma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanji","family":"Yamasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(98)00287-2"},{"key":"e_1_2_1_3_1","unstructured":"Endocrine Disruptor Screening and Testing Advisory Committee (EDSTAC).1998.Final report.http:\/\/www.epa.gov\/scipoly\/oscpendo\/history\/finalrpt.htm."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.854"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-83-20301"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.9.2579"},{"volume-title":"3rd Meeting of the Validation Management Group for the Screening and Testing of Endocrine Disrupters (Mammalian Effects)","year":"2001","author":"Organization of Economic Cooperation and Development (OECD)","key":"e_1_2_1_7_1"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-452806-2.50016-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(87)90199-3"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002040050651"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0780031"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.1038","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.1038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T16:35:47Z","timestamp":1760546147000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.1038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1002\/jat.1038"],"URL":"https:\/\/doi.org\/10.1002\/jat.1038","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"type":"print","value":"0260-437X"},{"type":"electronic","value":"1099-1263"}],"subject":[],"published":{"date-parts":[[2005,3]]}}}