{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T13:29:18Z","timestamp":1768483758322,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,2]],"date-time":"2005-03-02T00:00:00Z","timestamp":1109721600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The murine local lymph node assay (LLNA) is currently recognized as a stand\u2010alone sensitization test for determining the sensitizing potential of chemicals, and it has the advantage of yielding a quantitative endpoint that can be used to predict the sensitization potency of chemicals. The<jats:sc> ec<\/jats:sc><jats:sub>3<\/jats:sub> has been proposed as a parameter for classifying chemicals according to the sensitization potency. We previously developed a non\u2010radioisotopic endpoint for the LLNA based on 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU) incorporation (non\u2010RI LLNA), and we are proposing a new procedure to predict the sensitization potency of chemicals based on comparisons with known human contact allergens. Nine chemicals (i.e. diphencyclopropenone, <jats:italic>p<\/jats:italic>\u2010phenylenediamine, glutaraldehyde, cinnamicaldehyde, citral, eugenol, isopropyl myristate, propyleneglycol and hexane) categorized as human contact allergen classes 1\u20135 were tested by the non\u2010RI LLNA with the following reference allergens: 2,4\u2010dinitrochlorobenzene (DNCB) as a class 1 human contact allergen, isoeugenol as a class 2 human contact allergen and <jats:italic>\u03b1<\/jats:italic>\u2010hexylcinnamic aldehyde (HCA) as a class 3 human contact allergen. Consequently, nine test chemicals were almost assigned to their correct allergen class. The results suggested that the new procedure for non\u2010RI LLNA can provide correct sensitization potency data. Sensitization potency data are useful for evaluating the sensitization risk to humans of exposure to new chemical products. Accordingly, this approach would be an effective modification of LLNA with regard to its experimental design. Moreover, this procedure can be applied also to the standard LLNA with radioisotopes and to other modifications of the LLNA. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.1045","type":"journal-article","created":{"date-parts":[[2005,3,2]],"date-time":"2005-03-02T14:19:09Z","timestamp":1109773149000},"page":"129-134","source":"Crossref","is-referenced-by-count":33,"title":["Novel approach for classifying chemicals according to skin sensitizing potency by non\u2010radioisotopic modification of the local lymph node assay"],"prefix":"10.1002","volume":"25","author":[{"given":"Masahiro","family":"Takeyoshi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Iida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Shiraishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satsuki","family":"Hoshuyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-1873.2004.00278.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(92)90138-B"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(96)00059-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2000.042006344.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(00)01704-1"},{"key":"e_1_2_1_7_1","volume-title":"Computational Handbook of Statistics","author":"Bruning JL","year":"1997"},{"key":"e_1_2_1_8_1","first-page":"343","volume-title":"Methods in Immunotoxicology","author":"Buehler EV","year":"1995"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1263(199807\/08)18:4<281::AID-JAT506>3.0.CO;2-4"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/ajcd.2001.23926"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2003.07.001"},{"key":"e_1_2_1_12_1","first-page":"569","article-title":"Reduction, refinement and replacement: putting the immune system to work \u2014 the FRAME Annual Lecture 2002","volume":"30","author":"Kimber I","year":"2002","journal-title":"Altern. 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