{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:12:02Z","timestamp":1782785522595,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,10,11]],"date-time":"2005-10-11T00:00:00Z","timestamp":1128988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The murine local lymph node assay (LLNA) is currently recognized as a stand\u2010alone test method for determining the skin sensitizing potential of chemicals. It has been incorporated into the official test guidelines published by some authorities, including the OECD. To avoid the use of radioisotopes, efforts have been made recently to develop non\u2010radioisotopic modifications of the LLNA. A non\u2010radioisotopic modification of the LLNA was developed previously using 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU) incorporation (non\u2010RI LLNA). However, the non\u2010RI LLNA was found to be somewhat less sensitive than the standard assay. This study reports the advantage of using mice of the CBA\/N strain in the non\u2010RI LLNA to improve the sensitivity of this method. The non\u2010RI LLNA was performed using CBA\/JN and CBA\/N mice exposed to one of four confirmed skin sensitizers, 2,4\u2010dinitrochlorobenzene (DNCB), eugenol (EG), isoeugenol (IEG) or <jats:italic>\u03b1<\/jats:italic>\u2010hexylcinnamic aldehyde (HCA), and to one non\u2010sensitizer, propylene glycol (PG). The EC3 values for DNCB, IEG, EG, HCA and PG were calculated to be 0.1%, 9.6%, 40.6%, 45.5% and &gt;50% in CBA\/JN mice and 0.08%, 1.9%, 10.7%, 20.3% and &gt;50% in CBA\/N mice, respectively. The EC3 values for DNCB, IEG, EG, HCA and PG in the standard LLNA using CBA\/Ca mice and radioisotopes were reported elsewhere as being 0.08%, 1.3%, 13.0%, 8.0% and &gt;50%, respectively. The EC3 values derived from the CBA\/N mice in the non\u2010RI LLNA were nearly equivalent to the EC3 values obtained using the standard radioisotopic LLNA with CBA\/Ca mice. These data suggest that the use of CBA\/N mice may provide a realistic opportunity to develop a version of the LLNA that does not have a requirement for the use of radioisotopes, but which nevertheless has sensitivity approaching, or comparable to, the standard method. 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