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The Gln49\u2010PLA<jats:sub>2<\/jats:sub> obviously induced an increase of the pain threshold in intoxicated 615 mice compared with the control group, suggesting it is a neurotoxin. Hot\u2010plate tests also showed that its analgesic activity was dose\u2010dependent, and naloxone antagonized the analgesic effect, implying the mechanism of action of Gln49\u2010sPLA<jats:sub>2<\/jats:sub> is correlated with opioid receptors. Electrophysiology studies revealed decreases in the action potential and the nerve conduction velocity in isolated hoptoad (<jats:italic>Bufo bufo gargarizans<\/jats:italic> Cantor) sciatic nerve, indicating Gln49\u2010PLA<jats:sub>2<\/jats:sub> most probably had effects on ion channels. 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