{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:49:30Z","timestamp":1790732970558,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,6,22]],"date-time":"2007-06-22T00:00:00Z","timestamp":1182470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human prostate gland has low basal expression of the metallothionein\u20101 and \u20102 proteins. In prostate cancer, MT\u20101\/2 protein expression is variable and correlates directly with the increasing Gleason score of the tumor. The goal of the present study was to determine if the RWPE\u20101 cell line is a good model to elucidate the mechanisms underlying the alterations in MT\u20101\/2 expression that occur during the development of prostate cancer. It was shown that the RWPE\u20101 cell line and <jats:italic>in situ<\/jats:italic> prostate tissue have identical expression profiles of MT\u20101 and MT\u20102 isoform\u2010specific mRNAs (MT\u20101E, MT\u20101X and MT\u20102A) and similar levels of MT\u20101\/2 protein. It was also shown that the RWPE\u20101 cells respond to Zn<jats:sup>+2<\/jats:sup> and Cd<jats:sup>+2<\/jats:sup> exposure by induction of the basally expressed MT mRNAs and the accumulation of high levels MT\u20101\/2 protein (in excess of 10% of total protein). It was also shown that additional MT\u20101 mRNAs were expressed when the cells were exposed to either metal; MT\u20101A, MT\u20101F, MT\u2010G and MT\u20101H for Cd<jats:sup>+2<\/jats:sup>\u2010exposed cells; and, MT\u20101F, MT\u2010G and MT\u20101H for Zn<jats:sup>+2<\/jats:sup>\u2010exposed cells. The results suggest that RWPE\u20101 cells may be a valuable system to define the interplay between Zn<jats:sup>+2<\/jats:sup> concentration, Cd<jats:sup>+2<\/jats:sup> exposure and MT in normal and malignant prostate epithelial cells. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.1277","type":"journal-article","created":{"date-parts":[[2007,6,22]],"date-time":"2007-06-22T08:48:21Z","timestamp":1182502101000},"page":"283-293","source":"Crossref","is-referenced-by-count":33,"title":["Basal and metal\u2010induced expression of metallothionein isoform 1 and 2 genes in the RWPE\u20101 human prostate epithelial cell line"],"prefix":"10.1002","volume":"28","author":[{"given":"Amy L.","family":"Albrecht","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra K.","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seema","family":"Somji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Ann","family":"Sens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald A.","family":"Sens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott H.","family":"Garrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6,22]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/94.24.1888"},{"key":"e_1_2_1_3_1","first-page":"455","article-title":"Cadmium\u2010induced malignant transformation of human prostate epithelial cells","volume":"61","author":"Achanzar WE","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006\u20102952(99)00301\u20109"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.6.1215"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7832"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.11.017"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1994.1083"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2003.07.013"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00255877"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00254429"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(20000501)43:2<125::AID-PROS7>3.0.CO;2-S"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3434234"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:BIOM.0000045738.59708.20"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0710133"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.55.070186.004405"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00390356"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(01)18463\u20101"},{"key":"e_1_2_1_19_1","first-page":"119","volume-title":"Beryllium, Cadmium, Mercury and Exposures in the Glass Industry","author":"IARC","year":"1993"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102559.1997.2140848.x"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1172"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm902"},{"key":"e_1_2_1_23_1","first-page":"29","volume-title":"Metallothionein III. Biological Roles and Medical Implications","author":"K\u00e4gi JHR","year":"1993"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0680359"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.28-1637490"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.3109\/07435808409035418"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/07435808409035417"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.1976.tb23122.x"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002\u2010221054"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.143.1.8581"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0703\u2010239"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20104274(96)03632\u20106"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1442\u20102042.2002.00544.x"},{"key":"e_1_2_1_34_1"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/annhyg\/8.1.55"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287390390227651"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990190104"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102559.2004.01922.x"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1993.73.1.79"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287399409531920"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.6.1225"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20105347(01)65481\u20108"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.1277","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.1277","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:50:17Z","timestamp":1760529017000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.1277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,22]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["10.1002\/jat.1277"],"URL":"https:\/\/doi.org\/10.1002\/jat.1277","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,22]]}}}