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Current regulatory initiatives are driving the impetus for the use of <jats:italic>in vitro\/in silico<\/jats:italic> alternative approaches to provide the relevant information needed for the effective assessment of skin sensitisation, for both hazard characterisation and risk assessment purposes. A chemical must undergo a number of steps for it to induce skin sensitisation but the main determining step is formation of a stable covalent association with carrier protein. The ability of a chemical to react covalently with carrier protein nucleophiles relates to both its electrophilic reactivity and its hydrophobicity.<\/jats:p><jats:p>This paper focuses on quantitative indices of electrophilic reactivity with nucleophiles, in a chemical mechanism\u2010of\u2010action context, and compares and contrasts the experimental approaches available to generate reactivity data that are suitable for mathematical modelling and making predictions of skin sensitisation potential, using new chemistry data correlated against existing <jats:italic>in vivo<\/jats:italic> bioassay data. As such, the paper goes on to describe an illustrative example of how quantitative kinetic measures of reactivity can be usefully and simply applied to perform mechanism\u2010based read\u2010across that enables hazard characterisation of skin sensitisation potential. An illustration of the types of quantitative mechanistic models that could be built using databases of kinetic measures of reactivity, hydrophobicity and existing <jats:italic>in vivo<\/jats:italic> bioassay data is also given. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.1293","type":"journal-article","created":{"date-parts":[[2007,8,17]],"date-time":"2007-08-17T07:50:40Z","timestamp":1187337040000},"page":"443-454","source":"Crossref","is-referenced-by-count":65,"title":["Chemical reactivity indices and mechanism\u2010based read\u2010across for non\u2010animal based assessment of skin sensitisation potential"],"prefix":"10.1002","volume":"28","author":[{"given":"David W.","family":"Roberts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aynur O.","family":"Aptula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Patlewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla","family":"Pease","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2007.01.008"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15569520701212282"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx050075m"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2005.07.003"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx0601004"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx060102o"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.00944.x"},{"key":"e_1_2_1_9_1","first-page":"129","volume-title":"Allergic Contact Dermatitis. 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