{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T09:41:11Z","timestamp":1771753271501,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,5,25]],"date-time":"2010-05-25T00:00:00Z","timestamp":1274745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The intact female weanling version in the Organization for Economic Cooperation and Development (OECD) uterotrophic assay Test Guideline (TG) 440 is proposed as an alternative to the adult ovariectomized female version, because it does not involve surgical intervention (vs the ovariectomized version) and detects direct\/indirect\u2010acting estrogenic\/anti\u2010estrogenic substances (vs the ovariectomized version which detects only direct\u2010acting estrogenic\/anti\u2010estrogenic substances binding to the estrogen receptor). This validation study followed OECD TG 440, with six female weanling rats (postnatal day 21) per dose group and six treatment groups. Females were weighed and dosed once daily by oral gavage for three consecutive days, with one of six doses of 17<jats:italic>\u03b1<\/jats:italic>\u2010ethinyl estradiol in corn oil at 5\u2009ml\u2009kg<jats:sup>\u22121<\/jats:sup> at 0 and 0.1\u201310\u2009\u00b5g\u2009kg<jats:sup>\u22121<\/jats:sup> per day. On postnatal day 24, the juvenile females were euthanized by CO<jats:sub>2<\/jats:sub> asphyxiation, weighed, livers weighed and uteri weighed wet and blotted. The presence or absence of vaginal patency was recorded. Absolute and relative (to terminal body weight) uterine wet and blotted weights and uterine luminal fluid weights were significantly increased at 3.0 and 10.0 (both <jats:italic>P<\/jats:italic> &lt; 0.01) \u00b5g kg<jats:sup>\u22121<\/jats:sup> per day, and increased to \u223c140% of control values at 1.0\u2009\u00b5g\u2009kg<jats:sup>\u22121<\/jats:sup> per day (not statistically significantly). <jats:italic>In vivo<\/jats:italic> body weights, weight changes, feed consumption, liver weights and terminal body weights were unaffected. Vaginal patency was not acquired in any female at any dose, although vaginal puckering was observed in one female at 10.0\u2009\u00b5g\u2009kg<jats:sup>\u22121<\/jats:sup> per day. Therefore, this intact weanling uterotrophic assay is validated in our laboratory for use under US and European endocrine toxicity testing programs\/legislation. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.1544","type":"journal-article","created":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T21:56:17Z","timestamp":1274910977000},"page":"694-698","source":"Crossref","is-referenced-by-count":9,"title":["Validation of the intact rat weanling uterotrophic assay with notes on the formulation and analysis of the positive control chemical in vehicle"],"prefix":"10.1002","volume":"30","author":[{"given":"Rochelle W.","family":"Tyl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa C.","family":"Marr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherri S.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily A.","family":"Dolbow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina B.","family":"Myers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,10,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0031-6997(25)07146-7","article-title":"Pharmacological implications of microsomal enzyme induction","volume":"19","author":"Conney AH.","year":"1967","journal-title":"Pharmacol. 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Available from:http:\/\/eur\u2010lex\u2010europa.eu\/LexUriServ\/LexUriServ.do?uri=CoNSLEG:2006R1907:20071123:EN:pdforhttp:\/\/europa.eu\/environment\/chemicals\/reach\/reach_intro.htm"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.01109785"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/01621459.1952.10483441"},{"key":"e_1_2_6_9_1","first-page":"278","volume-title":"Contributions to Probability and Statistics","author":"Levene H.","year":"1960"},{"key":"e_1_2_6_10_1","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"NRC","year":"1996"},{"key":"e_1_2_6_11_1","unstructured":"Organization for Economic Cooperation and Development.2007.Guideline for Testing of Chemicals no. 440 \u2018Uterotrophic Bioassay in Rodents: a short\u2010term screening test for oestrogenic properties\u2019;1\u201321."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfp266"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/68.1.121"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfj203"},{"key":"e_1_2_6_15_1","article-title":"Prevention, pesticides and toxic substances. 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