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Instead of radioisotope using in this method, Takeyoshi M. <jats:italic>et\u2009al<\/jats:italic>. () has developed a modified LLNA based on the 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU) incorporation (LLNA:BrdU\u2010ELISA). The LLNA:BrdU\u2010ELISA is practically identical to the LLNA methodology excluding the use of BrdU, for which a single intraperitoneal injection of BrdU is made on day 4, and colorimetric detection of cell turnover. We conducted the validation study to evaluate the reliability and relevance of LLNA:BrdU\u2010ELISA.<\/jats:p><jats:p>The experiment involved 7 laboratories, wherein 10 chemicals were examined under blinded conditions. In this study, 3 chemicals were examined in all laboratories and the remaining 7 were examined in 3 laboratories. The data were expressed as the BrdU incorporation using an ELISA method for each group, and the stimulation index (SI) for each chemical\u2010treated group was determined as the increase in the BrdU incorporation relative to the concurrent vehicle control group. An SI of 2 was set as the cut\u2010off value for exhibiting skin sensitization activity.<\/jats:p><jats:p>The results obtained in the experiments conducted for all 10 chemicals were sufficiently consistent with small variations in their SI values. The sensitivity, specificity, and accuracy of LLNA:BrdU\u2010ELISA against those of GPMT\/BT were 7\/7 (100%), 3\/3 (100%), and 10\/10 (100%), respectively. 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