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In this study, we collected 1406 genes from previous microarray findings, validated changes in their expression by RT\u2010PCR analysis in local lymph nodes draining skin exposed to different sensitizers, and interpreted their biological function through pathway\u2010based genomic analysis, in which 468 genes were identified as being in the KEGG pathway database. The top\u2010ranked functions (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) identified as being affected by the sensitizers were associated with aspects of cell growth, such as DNA replication, cell cycle regulation and pyrimidine metabolism. All the sensitizers tested (DNCB, OXA and TDI) induced significant up\u2010regulation of <jats:italic>Psme4<\/jats:italic>, which is associated with DNA replication; <jats:italic>Tfdp1<\/jats:italic>, which is related to cell cycle regulation; and <jats:italic>Dut<\/jats:italic>, which is involved in pyrimidine metabolism. Specific changes were also shown in functional categories related to the immune response, including cytokines and their receptors. Genes identified in these functional categories, such as <jats:italic>Ccl21c<\/jats:italic>, <jats:italic>Cxcl9<\/jats:italic>, <jats:italic>Cxcl10<\/jats:italic>, <jats:italic>Ifng<\/jats:italic> and <jats:italic>Il12rb1<\/jats:italic>, were found to have functional relevance. These findings may enhance our understanding and assessment of chemical sensitizers, and enable us to distinguish sensitizers from irritants and to classify chemicals as contact sensitizers. 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