{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:56:06Z","timestamp":1760576166789,"version":"build-2065373602"},"reference-count":7,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":7670,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[1985,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Disulfiram, in 1 mg and 10 mg oral doses, was given to inbred C<jats:sub>3<\/jats:sub>H mice prior to and for the duration of pregnancy. The effects on the fetus have been assessed at 18 days gestation by means of fetal and placental weights, number of resorptions, skeletal preparations and Wilson's sections. Disulfiram dissolved in diethyl ether was administered to 8\u2010 and 9\u2010day embryos <jats:italic>in vitro<\/jats:italic> in concentrations of 0.1, 10 and 100 \u03bcgml<jats:sup>\u22121<\/jats:sup> of culture medium, and the effects of ether alone and ether plus disulfiram assessed by evaluation of morphological development over a 28\u2010h period, and inhibition of DNA synthesis using tritiated thymidine labelling over a 4\u2010h period. Disulfiram (1 mg) <jats:italic>in vivo<\/jats:italic> caused no adverse effects on the fetus, but disulfiram (10 mg) was toxic, in that it caused a significant increase in early resorptions. Disulfiram <jats:italic>in vitro<\/jats:italic>, in the 10 and 100 \u03bcg ml<jats:sup>\u22121<\/jats:sup> concentrations, proved to be very toxic to the embryos, affecting both morphological development and DNA synthesis in 9\u2010day embryos and morphological development in 8\u2010day embryos. DNA synthesis was only inhibited at the 100 \u03bcg ml<jats:sup>\u22121<\/jats:sup> concentration in 8\u2010day embryos. The 0.1 \u03bcg ml<jats:sup>\u22121<\/jats:sup> concentration of disulfiram caused abnormal central nervous system development in 8\u2010day embryos, but was otherwise non\u2010toxic to 8\u2010 and 9\u2010day embryos. Apart from a reduction in somite counts, ether in concentrations of 0.285 mg ml<jats:sup>\u22121<\/jats:sup> and 2.85 mg ml<jats:sup>\u22121<\/jats:sup> caused no adverse effects on morphological development in 8\u2010 or 9\u2010day embryos. DNA synthesis was inhibited by ether in a concentration of 2.85 mg ml<jats:sup>\u22121<\/jats:sup> in 9\u2010day embryos.<\/jats:p>","DOI":"10.1002\/jat.2550050102","type":"journal-article","created":{"date-parts":[[2006,10,14]],"date-time":"2006-10-14T14:27:27Z","timestamp":1160836047000},"page":"1-10","source":"Crossref","is-referenced-by-count":7,"title":["The effects of disulfiram on the experimental C<sub>3<\/sub>H mouse embryo"],"prefix":"10.1002","volume":"5","author":[{"given":"P. A. C.","family":"Thompson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. I.","family":"Folb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(77)92534-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0390375"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2550020404"},{"key":"e_1_2_1_5_2","unstructured":"P. A. C.Thompson A study of the pathogenesis of fetal damage caused by ethanol in the experimental mouse. Doctoral Thesis University of Cape Town (1982)."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051270303"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10520293809111373"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1978.tb00993.x"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.2550050102","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.2550050102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:09:31Z","timestamp":1760548171000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.2550050102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,2]]},"references-count":7,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985,2]]}},"alternative-id":["10.1002\/jat.2550050102"],"URL":"https:\/\/doi.org\/10.1002\/jat.2550050102","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"type":"print","value":"0260-437X"},{"type":"electronic","value":"1099-1263"}],"subject":[],"published":{"date-parts":[[1985,2]]}}}