{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:38:46Z","timestamp":1790483926709,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,1,11]],"date-time":"2006-01-11T00:00:00Z","timestamp":1136937600000},"content-version":"vor","delay-in-days":5581,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[1990,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sodium tetrathionate has been proposed as a cyanide antidote despite its reported toxicology and inhibitory effect on rhodanese. We investigated the effectof tetrathionate and an analog, dithionite, on rhodanese activity because of their structural similarily to thiosulfate, a known sulfane sulfur donor for this enzyme. Rhodanese activity of guinea pig liver homogenate was assayed by measuring the formation of ferric thicyanate complex at 460 nm. With thiosulfate as a substrate, the <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> for rhodanese was 6.7 mM and the <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> was 0.67 \u03bcmol thiocyanate min<jats:sup>\u22121<\/jats:sup> mg<jats:sup>\u22121<\/jats:sup> protein. The conversion of cyanide to thiocyanate by rhodanese was inhibited in the presence of terathionate at the millimolar concentration (e.g. 1 mM) range. Dithionite had a negligible effect on rhodanese activity. Netiher thiosulfate (1\u2013100 mM) nor dithionite produced significant amounts of thiocyanate from cyanide spontaneously (i.e. non\u2010enzymatically). However, in the absence of rhodanese, tetrathionate (1.0, 10.0 and 100.0 mM) produced a dose\u2010dependent increase in thiocyanate. These data suggest that tetrathionate detoxifies cyanide non\u2010enzymatically, which may, in part, account for its antidotal effects.<\/jats:p>","DOI":"10.1002\/jat.2550100511","type":"journal-article","created":{"date-parts":[[2006,10,16]],"date-time":"2006-10-16T01:59:22Z","timestamp":1160963962000},"page":"379-382","source":"Crossref","is-referenced-by-count":7,"title":["The effect of sodium tetrathionate on cyanide conversion to thiocyanate by enzymatic and non\u2010enzymatic mechanisms"],"prefix":"10.1002","volume":"10","author":[{"given":"Steven I.","family":"Baskin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen D.","family":"Kirby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470513712.ch12"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1016\/S0021-9258(19)76995-6","article-title":"Mechanisms of sulfur transfer catalysis. Sulfhydryl\u2010catalyzed transfer of thionsulfonate sulfur","volume":"246","author":"Westley J.","year":"1971","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_4_2","first-page":"383","volume-title":"Clinical and Experimental Toxicology of Cyanides","author":"Marrs T. C.","year":"1987"},{"key":"e_1_2_1_5_2","first-page":"1941","article-title":"Contribution to the study of hydrocyanic poisoning: antidotal value of sodium tetrathionate","volume":"226","author":"Binet L.","year":"1948","journal-title":"C. R. Acad. Sci."},{"key":"e_1_2_1_6_2","first-page":"1781","article-title":"The mechanism of the antidotal action of sodium tetrathionate against hydocyanic poisoning","volume":"228","author":"Binet L.","year":"1949","journal-title":"C. R. Acad. Sci."},{"key":"e_1_2_1_7_2","first-page":"641","article-title":"The value and the mechanism of the antidotal action of sodium tetrathionate in hydrocyanic poisoning","volume":"59","author":"Binet L.","year":"1951","journal-title":"Presse. med."},{"key":"e_1_2_1_8_2","first-page":"140","article-title":"Sodium tetrathionate as antidote in hydrocyanic acid poisonings","volume":"42","author":"Chistoni A.","year":"1932","journal-title":"Arch. Int. Pharmacol. Ther."},{"key":"e_1_2_1_9_2","first-page":"439","article-title":"Potassium polythionates as antidotes for cyanide poisoning","volume":"49","author":"Chistoni A.","year":"1935","journal-title":"Arch. Int. Pharmacol. 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