{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T03:59:40Z","timestamp":1772337580167,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,1,11]],"date-time":"2006-01-11T00:00:00Z","timestamp":1136937600000},"content-version":"vor","delay-in-days":4850,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Investigations to determine the inhibitory activity on the Ca<jats:sup>2+<\/jats:sup>\u2010transport\u2010ATPase of human erythrocyte membranes were performed with various compounds of toxicological significance, mostly chlorinated and mainly used as biocides, such as phenol, 4\u2010chlorophenol. 2,4\u2010dichlorophenol, 2,6\u2010dichlorophenol, 3,4\u2010dichlorophcnol, 2,3,4\u2010trichlorophenol, 2,3,4,5\u2010tetrachlorophenol, pentachlorophenol (PCP), captan, folpet, captafol, (+)\u2010camphene, toxaphene, dichlorodiphenyltrichloroethane (UDT), lindane, endrin, dieldrin, \u03b1\u2010endosulfan, \u03b2\u2010endosulfan, paraquat, diallate, 2,4\u2010dichlorophenoxyaceticacid (2,4\u2010D) and 2,4,5\u2010trichlorophenoxyacetic acid (2,4,5\u2010T).<\/jats:p><jats:p>Some of the compounds investigated display an inhibitory effect on the Ca<jats:sup>2+<\/jats:sup>\u2010transport\u2010ATPase at very low concentrations. The <jats:italic>in vitro<\/jats:italic> results obtained in this enzyme assay can be correlated directly with the results of other <jats:italic>in vitro<\/jats:italic> assays and with the results of <jats:italic>in vivo<\/jats:italic> investigations in different species in whkh an inhibitory effect on various biological functions is observed. Therefore, an inhibitory effect on the Ca<jats:sup>2+<\/jats:sup>\u2010transport\u2010ATPase indicates a toxic effect of these compounds to cell functions.<\/jats:p><jats:p>Since the inhibitory effect of these compounds can be measured rapidly and the enzyme is easy to handle, it might be a useful tool to screen the toxic effects of various compounds on cell function. The aim of the authors was to investigate the usefulness of this screening test system for the characterization of the cellular toxicity of various compounds.<\/jats:p>","DOI":"10.1002\/jat.2550120511","type":"journal-article","created":{"date-parts":[[2006,10,14]],"date-time":"2006-10-14T12:37:07Z","timestamp":1160829427000},"page":"351-358","source":"Crossref","is-referenced-by-count":20,"title":["The Ca<sup>2+<\/sup>\u2010transport\u2010atpase of human erythrocytes as an <i>in vitro<\/i> toxicity test system\u2014acute effects of some chlorinated compounds"],"prefix":"10.1002","volume":"12","author":[{"given":"F.","family":"Janik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. U.","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1880047"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1016\/S0021-9258(17)33231-3","article-title":"Structural similarities between the Ca2+\u2010dependent regulatory proteins of 3:5\u2010cyclic nucleotide phosphodiesterase and actomyosin ATPase","volume":"251","author":"Watterson D. M.","year":"1976","journal-title":"J. Biol. 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Pharmacol. Suppl."},{"key":"e_1_2_1_19_2","first-page":"76","article-title":"Mammalian spermatozoa as in vitro model for studies on acute cytotoxicity of chemicals","volume":"332","author":"Seibert H.","year":"1986","journal-title":"Naunyn\u2010Schmeideberg's Arch. Pharmacol. Suppl."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(81)91656-9"},{"key":"e_1_2_1_21_2","first-page":"20","article-title":"The biological action of chlorophenols","volume":"13","author":"Farquharson M. E.","year":"1957","journal-title":"Br. J. Pharmacol."},{"key":"e_1_2_1_22_2","unstructured":"F.Janik Die Wirkung von Bioziden auf die Aktivit\u00e4t der Ca2+\u2010Transport\u2010ATPase von Humanerythrozyten und der m\u00f6gliche Ensatz dieses Enzyms in einem Testsystem zur Erfassung der akuten Toxizit\u00e4t bestimmter chemischer Substanzen. Dissertation Ulm Germany (1987)."}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.2550120511","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.2550120511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T03:30:21Z","timestamp":1760585421000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.2550120511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,10]]}},"alternative-id":["10.1002\/jat.2550120511"],"URL":"https:\/\/doi.org\/10.1002\/jat.2550120511","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10]]}}}