{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:53:45Z","timestamp":1790232825319,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,11]],"date-time":"2006-01-11T00:00:00Z","timestamp":1136937600000},"content-version":"vor","delay-in-days":4758,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Brain mitochondrial cytochrome oxidase and respiratory activities were compared after\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    exposure to cyanide. For the\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies, mice were exposed to a non\u2010lethal (4 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) or lethal (20 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) dose of KCN. From these mice, purified brain mitochondria were prepared and cytochrome oxidase and respiratory activities measured. Results of these experiments revealed greater inhibition of cytochrome oxidase activity following a lethal (20 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) than a non\u2010lethal (4 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) KCN dose (57 and 45% inhibition, respectively). Respiration states 3 and 4 of brain mitochondria prepared from mice that received 4 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    KCN were inhibted by 15 and 20%, respectively. In mice that received a lethal 20 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    KCN dose, respiration states 3 and 4 were each inhibited by ca. 30% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05).\n                    <jats:italic>In vitro<\/jats:italic>\n                    , mitochondrial cytochrome oxidase activity was inhibited in a concentration\u2010dependent fashion at cyanide concentrations of 10\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u201310\n                    <jats:sup>\u22122<\/jats:sup>\n                    M. A biphasic inhibition of ADP\u2010stimulated (state 3) respiration was observed. Cyanide concentrations of 10\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u201310\n                    <jats:sup>\u22124<\/jats:sup>\n                    M produced only a 25% inhibition of respiration state 3, whereas 10\n                    <jats:sup>\u22123<\/jats:sup>\n                    M produced 80% inhibition. Because this dramatic inhibition only occurred at cyanide concentrations that caused &gt;50% inhibition of mitochondrial cytochrome oxidase activity, these findings suggest that a large proportion of cytochrome oxidase activity may be functional reserve and that cyanide poisoning likely involves other mechanisms in addition to inhibition of cytochrome oxidase.\n                  <\/jats:p>","DOI":"10.1002\/jat.2550130104","type":"journal-article","created":{"date-parts":[[2006,10,16]],"date-time":"2006-10-16T03:36:59Z","timestamp":1160969819000},"page":"9-14","source":"Crossref","is-referenced-by-count":42,"title":["The effects of cyanide on brain mitochondrial cytochrome oxidase and respiratory activities"],"prefix":"10.1002","volume":"13","author":[{"given":"John C.","family":"Pettersen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"109","article-title":"An experimental assessment of the diagnostic potential of histochemical and biochemical methods for cytochrome oxidase in acute cyanide poisoning","volume":"22","author":"Ballantyne B.","year":"1977","journal-title":"Cell. 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