{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:58:25Z","timestamp":1760576305246,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,11]],"date-time":"2006-01-11T00:00:00Z","timestamp":1136937600000},"content-version":"vor","delay-in-days":4393,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of isoflurane on the anaerobic metabolism of halothane to chlorodifluoroethene (CDE) and chlorotrifluoroethane (CTE) was studied with microsomes of guinea pig liver by gas chromatography. The reaction mixture used to measure the end products of anaerobic metabolism consisted of a microsomal suspension, 3 mM NADPH, halothane and isoflurane (except in control groups) in 0.1 M potassium phosphate buffer solution (pH 7.4). The <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> values for CDE formation were 601.61 \u00b1 266.91, 254.22 \u00b1 86.58, 257.92 \u00b1 129.11, 268.55 \u00b1 125.66 and 319.22 \u00b1 86.76 \u03bcM (mean \u00b1 SD, <jats:italic>n<\/jats:italic> = 5) at 0 mM (0%), 0.12 mM (0.26%), 0.29 mM (0.64%), 0.58 mM (1.30%) and 1.16 mM (2.59%) isoflurane, respectively. The <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> values for CTE formation were 1204.74 \u00b1 551.64, 553.75 \u00b1 177.89, 521.14 \u00b1 249.77, 560.67 \u00b1 229.61 and 711.05 \u00b1 317.13 \u03bc (<jats:italic>n<\/jats:italic> = 5) at 0 mM (0%), 0.12 mM (0.26%), 0.29 mM (0.64%), 0.58 mM (1.30%) and 1.16 mM (2.59%) isoflurane, respectively. In contrast, the <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> values for CDE and CTE formation at these isoflurane concentrations were not significantly different than in the control groups. In this study the production of CDE and CTE was significantly (<jats:italic>P<\/jats:italic> &lt; 0.05) increased by isoflurane, at concentrations up to 0.58 mM (1.30%).<\/jats:p>","DOI":"10.1002\/jat.2550140109","type":"journal-article","created":{"date-parts":[[2006,10,16]],"date-time":"2006-10-16T04:34:36Z","timestamp":1160973276000},"page":"43-46","source":"Crossref","is-referenced-by-count":1,"title":["Isoflurane increases the anaerobic metabolites of halothane"],"prefix":"10.1002","volume":"14","author":[{"given":"Mustafizur","family":"Rahman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohyu","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyoshi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osafumi","family":"Yuge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-196301000-00005"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196303072681004"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-196707000-00018"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(81)91569-2"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1093\/oxfordjournals.jbchem.a133704","article-title":"A spin trap study on anaerobic dehalogenation of halothane by a reconstituted liver microsomal cytochrome P\u2010450 enzyme system","volume":"91","author":"Fujii K.","year":"1982","journal-title":"J. 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