{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T15:55:37Z","timestamp":1769097337527,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,11]],"date-time":"2006-01-11T00:00:00Z","timestamp":1136937600000},"content-version":"vor","delay-in-days":3969,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This new bioassay determined the toxicity of chemical compounds dissolved in water by measuring the degree of inhibition of the ultraviolet light\u2010stimulated fluorescence of <jats:italic>Escherichia coli<\/jats:italic> in a culture medium in which 4\u2010methylumbelliferyl \u03b2\u2010D\u2010glucuronide was the only carbon source. Inhibition produced by one of five heavy\u2010metal salts (Cd<jats:sup>2+<\/jats:sup>, Cr<jats:sup>6+<\/jats:sup>, Hg<jats:sup>2+<\/jats:sup>, Pb<jats:sup>2+<\/jats:sup> or Zn<jats:sup>2+<\/jats:sup>) was the end\u2010point and comparison standard to determine the EC<jats:sub>50<\/jats:sub> and minimum effective concentration (MEC) that produced a decrease of <jats:italic>E. coli<\/jats:italic> growth rate, increased doubling time and percentage inhibition and reduced numbers of generations; all these values were derived from the fluorescence signals. Only Cr<jats:sup>6+<\/jats:sup> and Hg<jats:sup>2+<\/jats:sup> at two concentrations (0.25 and 0.5 mg I<jats:sup>\u22121<\/jats:sup>) almost completely inhibited this <jats:italic>E. coli<\/jats:italic> strain. All toxicant concentrations tested produced at least partial inhibitions of growth; Cr<jats:sup>6+<\/jats:sup>, Hg<jats:sup>2+<\/jats:sup> and Cd<jats:sup>2+<\/jats:sup>, in that order, were most toxic, and Pb<jats:sup>2+<\/jats:sup> the least. Zn<jats:sup>2+<\/jats:sup> gave higher EC<jats:sub>50<\/jats:sub> values at 3 h of incubation than at 4 h. The method was simple, rapid and inexpensive and would permit a large number of samples to be tested quickly.<\/jats:p>","DOI":"10.1002\/jat.2550150208","type":"journal-article","created":{"date-parts":[[2006,10,16]],"date-time":"2006-10-16T05:33:05Z","timestamp":1160976785000},"page":"103-107","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of the toxicity of several heavy metals by a fluorescent bacterial bioassay"],"prefix":"10.1002","volume":"15","author":[{"given":"Alberto","family":"Mariscal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Carnero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso","family":"Pinedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaqu\u00edn","family":"Fern\u00e1ndez\u2010Crehuet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,11]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Orgenics Ltd. The Toxi\u2010Chromotest(Version 2). PO Box 360 Yavne 70650 Israel (1985)."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2550100611"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"3006","DOI":"10.1128\/aem.56.10.3006-3016.1990","article-title":"Biotoxicity of mercury as influenced by mercury (II) speciation","volume":"56","author":"Farrell R. E.","year":"1990","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01963647"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.2166\/wst.1989.0116","article-title":"Cytochrome oxidase and O\u2010nitro\u2010phenyl\u2010\u03b2\u2010D\u2010galactopyranoside for improved and rapid confirmation of coliforms enumerated by the membrane filtration method","volume":"21","author":"Morgan P. G.","year":"1989","journal-title":"Water Sci. Technol."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(83)90010-9"},{"key":"e_1_2_1_8_2","first-page":"888","article-title":"New toxicity determination method that uses fluorescent assay of Escherichia coli","volume":"16","author":"Mariscal A.","year":"1994","journal-title":"Biotechnology"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2550090610"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/tox.2530070105"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02097772"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/978-1-4615-8945-7_6","volume-title":"Active sites of biological macromolecules and their interaction with heavy metals. Ecological Toxicology Research: Effects of Heavy Metal and Organohalogen Compounds","author":"Eichorn G. L.","year":"1975"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-6513(91)90040-V"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-6513(91)90020-P"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/tox.2530070104"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/tox.2530060404"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-6513(91)90070-6"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0269-7491(90)90097-V"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-6513(87)90077-7"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-6513(91)90043-O"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-6513(91)90040-V"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/tox.2540050404"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(89)90099-7"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00212137"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00194665"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.2550150208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.2550150208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:40:03Z","timestamp":1760550003000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.2550150208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1002\/jat.2550150208"],"URL":"https:\/\/doi.org\/10.1002\/jat.2550150208","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]}}}