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The chromatographic process was carried out using an XTerra\u00ae RP8 column (250\u2009\u00d7\u20094.6\u2009mm i.d., 5\u2009\u00b5m particle size) and a mobile phase composed of acetonitrile and pH 6.5 phosphate buffer in gradient mode. A linear response from the detector was obtained within the concentration range of 0.1\u20134\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup>, with correlation coefficients higher than 0.99. The limits of detection were lower than 30\u2009ng ml<jats:sup>\u22121<\/jats:sup> for all the drugs studied, the coefficients of variation fluctuated between 0.1 and 12.4%, and the average recoveries were higher than 78% for all the drugs except for EDDP, with a value of 66.4%. Finally, the proposed method was applied to 15 vitreous humor samples coming from individuals who had died from opiate and\/or cocaine overdose, showing consumption of cocaine in 14 cases, methadone in five cases and heroin in three cases. Average concentrations of 0.30\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for morphine, 0.24\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for 6\u2010acetylmorphine, 0.10\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for codeine, 0.81\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for cocaine, 1.26\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for benzoylecgonine, 0.15\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for cocaethylene, 0.11\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for methadone and 0.68\u2009\u00b5g ml<jats:sup>\u22121<\/jats:sup> for EDDP were obtained. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.2722","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T07:50:41Z","timestamp":1329292241000},"page":"740-745","source":"Crossref","is-referenced-by-count":20,"title":["Chromatographic determination of drugs of abuse in vitreous humor using solid\u2010phase extraction"],"prefix":"10.1002","volume":"33","author":[{"given":"Purificaci\u00f3n","family":"Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Institute of Forensic Sciences Forensic Toxicology Service, Faculty of Medicine  15782\u2010Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Seoane","sequence":"additional","affiliation":[{"name":"Institute of Forensic Sciences Forensic Toxicology Service, Faculty of Medicine  15782\u2010Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Institute of Forensic Sciences Forensic Toxicology Service, Faculty of Medicine  15782\u2010Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Tabernero","sequence":"additional","affiliation":[{"name":"Institute of Forensic Sciences Forensic Toxicology Service, Faculty of Medicine  15782\u2010Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia M.","family":"Carro","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemistry  15782\u2010Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa A.","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemistry  15782\u2010Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,2,15]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/31.8.469"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.12.041"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104347(96)00088\u20106"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104274(98)00292\u20106"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/31.8.537"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010007\u20101238\u20107"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079408013375"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.2002\/jat.1130"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS15227J"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/19.6.359"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/157341206776819346"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS12964J"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/24.1.59"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/24.7.458"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00769\u2010002\u20100516\u20105"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/23.3.168"},{"key":"e_1_2_4_18_1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1093\/clinchem\/39.3.481","article-title":"Cocaine and benzoylecgonine in saliva, serum and urine","volume":"39","author":"Schramm W","year":"1993","journal-title":"Clin. 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