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In the present study, zebrafish embryos and larvae were used as a model to investigate the sublethal and lethal effects of three different organochlorine pesticides, namely methoxychlor, endosulfan and heptachlor, as well as the flame retardant tetrabromobisphenol A, and its precursor compound bisphenol A. Preliminary data for chemical exposure tests were obtained by determining the 96\u2009h median effective concentration EC<jats:sub>50<\/jats:sub> (hatching rate) and 96\u2009h median lethal concentration LC<jats:sub>50<\/jats:sub>. Quantitative polymerase chain reaction was used to investigate the gene expression levels of the biomarker vitellogenin (<jats:italic>vtg1<\/jats:italic>) after 96\u2009h exposures to 10, 25, 50 and 75% of the 96\u2009h EC<jats:sub>50<\/jats:sub> value for embryos and 96\u2009h LC<jats:sub>50<\/jats:sub> value for larvae. The use of <jats:italic>vtg1<\/jats:italic> mRNA induction in zebrafish embryos and larvae was found to be a sensitive biomarker of exposure to these organic compounds, and was helpful in elucidating their adverse effects and setting water quality guidelines. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.2723","type":"journal-article","created":{"date-parts":[[2012,2,21]],"date-time":"2012-02-21T02:02:28Z","timestamp":1329789748000},"page":"670-678","source":"Crossref","is-referenced-by-count":89,"title":["Toxicity assessment and vitellogenin expression in zebrafish (<i>Danio rerio<\/i>) embryos and larvae acutely exposed to bisphenol A, endosulfan, heptachlor, methoxychlor and tetrabromobisphenol A"],"prefix":"10.1002","volume":"33","author":[{"given":"Wing Shan","family":"Chow","sequence":"first","affiliation":[{"name":"Biochemistry Program, School of Life Sciences, Faculty of Science The Chinese University of Hong Kong  Sha Tin, N.T. 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