{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:35:12Z","timestamp":1787769312024,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,6,13]],"date-time":"2012-06-13T00:00:00Z","timestamp":1339545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A reactive oxygen species (ROS) assay was previously developed for photosafety evaluation of pharmaceuticals, and the present multi\u2010center study aimed to establish and validate a standard protocol for ROS assay. In three participating laboratories, two standards and 42 coded chemicals, including 23 phototoxins and 19 nonphototoxic drugs\/chemicals, were assessed by the ROS assay according to the standardized protocol. Most phototoxins tended to generate singlet oxygen and\/or superoxide under UV\u2013vis exposure, but nonphototoxic chemicals were less photoreactive. In the ROS assay on quinine (200\u2009\u00b5\n                    <jats:sc>m<\/jats:sc>\n                    ), a typical phototoxic drug, the intra\u2010 and inter\u2010day precisions (coefficient of variation; CV) were found to be 1.5\u20137.4% and 1.7\u20139.3%, respectively. The inter\u2010laboratory CV for quinine averaged 15.4% for singlet oxygen and 17.0% for superoxide. The ROS assay on 42 coded chemicals (200\u2009\u00b5\n                    <jats:sc>m<\/jats:sc>\n                    ) provided no false negative predictions upon previously defined criteria as compared with the\n                    <jats:italic>in vitro\/in vivo<\/jats:italic>\n                    phototoxicity, although several false positives appeared. Outcomes from the validation study were indicative of satisfactory transferability, intra\u2010 and inter\u2010laboratory variability, and predictive capacity of the ROS assay. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/jat.2776","type":"journal-article","created":{"date-parts":[[2012,6,13]],"date-time":"2012-06-13T23:57:45Z","timestamp":1339631865000},"page":"1241-1250","source":"Crossref","is-referenced-by-count":51,"title":["Establishment and intra\u2010\/inter\u2010laboratory validation of a standard protocol of reactive oxygen species assay for chemical photosafety evaluation"],"prefix":"10.1002","volume":"33","author":[{"given":"Satomi","family":"Onoue","sequence":"first","affiliation":[{"name":"Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences University of Shizuoka  52\u20101 Yada, Suruga\u2010ku Shizuoka 422\u20108526 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Hosoi","sequence":"additional","affiliation":[{"name":"Non\u2010clinical Research Group, Ophthalmic Research and Development Center, Santen Pharmaceutical Co. Ltd  8916\u201016 Takayama\u2010cho Ikoma Nara 630\u20100101 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinobu","family":"Wakuri","sequence":"additional","affiliation":[{"name":"Labolatory of Cell Toxicology, Hatano Research Institute, Food and Drug Safety Center  729\u20105 Ochiai Hadano Kanagawa 257\u20108523 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Iwase","sequence":"additional","affiliation":[{"name":"Safety Research Laboratories, Mitsubishi Tanabe Pharma Corporation  1\u20101\u20101, Kazusakamatari Kisarazu Chiba 292\u20100818 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshinobu","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Safety Research Laboratories, Mitsubishi Tanabe Pharma Corporation  2\u20102\u201050, Kawaguchi Toda\u2010shi Saitama 335\u20108505 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Safety Research Laboratories, Mitsubishi Tanabe Pharma Corporation  2\u20102\u201050, Kawaguchi Toda\u2010shi Saitama 335\u20108505 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuichi","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Product Development Regulatory Affairs Department, Shionogi &amp; Co. Ltd  2\u201017\u20105 Shibuya Shibuya\u2010ku Tokyo 150\u20108673 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuguto","family":"Toda","sequence":"additional","affiliation":[{"name":"Drug Developmental Research Laboratories, Shionogi &amp; Co. Ltd  3\u20101\u20101 Futaba\u2010cho Toyonaka Osaka 561\u20100825 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hironori","family":"Takagi","sequence":"additional","affiliation":[{"name":"Research Center, Taisho Pharmaceutical Co. Ltd  1\u2010403, Yoshino\u2010cho Kita\u2010ku Saitama 331\u20109530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Osaki","sequence":"additional","affiliation":[{"name":"Research Center, Taisho Pharmaceutical Co. Ltd  1\u2010403, Yoshino\u2010cho Kita\u2010ku Saitama 331\u20109530 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Safety Research Department, ASKA Pharmaceutical Co. Ltd  5\u201036\u20101, Shimosakunobe, Takatsu\u2010ku Kawasaki Kanagawa 213\u20108522 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Pharmaceuticals Research Center, Asahi Kasei Pharma Corporation  632\u20101 Mifuku Izunokuni\u2010shi Shizuoka 410\u20102321 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Seto","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences University of Shizuoka  52\u20101 Yada, Suruga\u2010ku Shizuoka 422\u20108526 Japan"},{"name":"Pharmacokinetics and Safety Research Department, Central Research Laboratories, Kaken Pharmaceutical Co. Ltd.  301 Gensuke Fujieda\u2010shi Shizuoka 426\u20108646 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences University of Shizuoka  52\u20101 Yada, Suruga\u2010ku Shizuoka 422\u20108526 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuo","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences University of Shizuoka  52\u20101 Yada, Suruga\u2010ku Shizuoka 422\u20108526 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Ohno","sequence":"additional","affiliation":[{"name":"National Institute of Health Sciences  1\u201018\u20101 Kamiyoga Setagaya\u2010ku Tokyo 158\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Kojima","sequence":"additional","affiliation":[{"name":"Japanese Center for the Validation of Alternative Methods, National Institute of Health Sciences  1\u201018\u20101 Kamiyoga Setagaya\u2010ku Tokyo 158\u20108501 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(00)00100-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S1383-5742(03)00052-8","article-title":"Photochemical genotoxicity: principles and test methods. Report of a GUM task force","volume":"566","author":"Brendler\u2010Schwaab S","year":"2004","journal-title":"Mutat. Res."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2003.00066.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-198530010-00005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2009.04.005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2009.11.006"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja809039u"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/b702752d"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200225050-00004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1989.tb04708.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-005-8497-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.09.084"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2007.09.003"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.03.026"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-007-9383-4"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488609788173044"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-010-0161-3"},{"key":"e_1_2_6_19_1","first-page":"415","article-title":"In vitro phototoxicity testing: development and validation of a new concentration response analysis software and biostatistical analyses related to the use of various prediction models","volume":"30","author":"Peters B","year":"2002","journal-title":"ATLA"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0781.2002.180207.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.22741"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480549609002201"},{"key":"e_1_2_6_23_1","first-page":"1031","article-title":"Evaluation of phototoxic properties of oral antidiabetics and diuretics. Photohemolysis model as a screening method for recognizing potential photosensitizing drugs","volume":"47","author":"Selvaag E","year":"1997","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-011-9292-7"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1969.tb07287.x"},{"key":"e_1_2_6_26_1","first-page":"314","article-title":"In vitro phototoxicity testing. The report and recommendations of ECVAM Workshop 2","volume":"22","author":"Spielmann H","year":"1994","journal-title":"ATLA"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0887-2333(94)90069-8"},{"key":"e_1_2_6_28_1","first-page":"22","article-title":"First results of an EC\/COLIPA validation project of in vitro phototoxicity testing methods","volume":"11","author":"Spielmann H","year":"1994","journal-title":"ALTEX"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000424321"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(98)00006-X"},{"key":"e_1_2_6_31_1","first-page":"679","article-title":"A study on UV filter chemicals from Annex VII of Euroepan Union Directive 76\/768\/EEC, in the in vitro 3\u2009T3 NRU phototoxicity test","volume":"26","author":"Spielmann H","year":"1998","journal-title":"ATLA"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1517\/14740338.6.4.431"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp0600182"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1159\/000246784"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2009.01.008"}],"container-title":["Journal of Applied Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjat.2776","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jat.2776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T22:06:16Z","timestamp":1760565976000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jat.2776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,13]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1002\/jat.2776"],"URL":"https:\/\/doi.org\/10.1002\/jat.2776","archive":["Portico"],"relation":{},"ISSN":["0260-437X","1099-1263"],"issn-type":[{"value":"0260-437X","type":"print"},{"value":"1099-1263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,13]]}}}