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Results of the nuclear morphology and the flow cytometer indicated that AMA efficiently induced PC12 cell apoptosis. Moreover, the levels of ROS and Ca<jats:sup>2+<\/jats:sup> increased in the early stage of cell apoptosis induced by AMA treatment. All of Ca<jats:sup>2+<\/jats:sup> chelators, L\u2010type Ca<jats:sup>2+<\/jats:sup> channel blockers and inhibitors of Ca<jats:sup>2+<\/jats:sup> released from endoplasmic reticulum and ROS scavenger, were used in this experiment. It was found that the Ca<jats:sup>2+<\/jats:sup> chelators and ROS scavengers, in particular, could delay AMA\u2010induced PC12 cell apoptosis. In conclusion, the present study found that AMA induced PC12 cell apoptosis through ROS and Ca<jats:sup>2+<\/jats:sup>. 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