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To assess the effects of acrylamide to freshwater fish, goldfish (<jats:italic>Carassius auratus<\/jats:italic> L.) were exposed to several concentrations of waterborne acrylamide and analysed for genotoxic damage, alterations to detoxifying enzymes and histopathology. Results revealed a dose\u2010dependent increase in total DNA strand breakage, the formation of erythrocytic nuclear abnormalities and in the levels of hepatic cytochrome P4501A (CYP1A) and glutathione S\u2010transferase (GST) activity. In addition, acrylamide induced more histopathological changes to pancreatic acini than to the hepatic parenchyma, regardless of exposure concentration, whereas hepatic tissue only endured significant alterations at higher concentrations of exposure. Thus, results confirm the genotoxic potential of acrylamide to fish and its ability to induce CYP1A, probably as a direct primary defence mechanism. This strongly suggests the substance's pro\u2010mutagenic potential in fish, similarly to what is known for rodents. However, the deleterious effects observed in the pancreatic acini, more severe than in the liver, could indicate a specific, albeit unknown toxic mechanism of acrylamide to fish that overran the organism's metabolic defences against a chemical agent rather than causing a general systemic failure. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.2936","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:42:09Z","timestamp":1381531329000},"page":"1293-1302","source":"Crossref","is-referenced-by-count":26,"title":["Histopathological findings on <i>Carassius auratus<\/i> hepatopancreas upon exposure to acrylamide: correlation with genotoxicity and metabolic alterations"],"prefix":"10.1002","volume":"34","author":[{"given":"Miguel","family":"Larguinho","sequence":"first","affiliation":[{"name":"CIGMH, Departamento Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  Campus de Caparica 2829\u2010516 Caparica Portugal"},{"name":"REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  Campus de Caparica 2829\u2010516 Caparica Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro M.","family":"Costa","sequence":"additional","affiliation":[{"name":"IMAR \u2013 Instituto do Mar, Departamento de Ci\u00eancias e Engenharia do Ambiente, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  2829\u2010516 Caparica Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gon\u00e7alo","family":"Sousa","sequence":"additional","affiliation":[{"name":"CIGMH, Departamento Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  Campus de Caparica 2829\u2010516 Caparica Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria H.","family":"Costa","sequence":"additional","affiliation":[{"name":"IMAR \u2013 Instituto do Mar, Departamento de Ci\u00eancias e Engenharia do Ambiente, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  2829\u2010516 Caparica Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rio S.","family":"Diniz","sequence":"additional","affiliation":[{"name":"REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  Campus de Caparica 2829\u2010516 Caparica Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro V.","family":"Baptista","sequence":"additional","affiliation":[{"name":"CIGMH, Departamento Ci\u00eancias da Vida, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa  Campus de Caparica 2829\u2010516 Caparica Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.21.841"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887\u20102333(98)00051\u20104"},{"key":"e_1_2_6_4_1","first-page":"291","article-title":"Application of acrylamide polymers as flocculants in sewages coagulation process","volume":"2","author":"Bajdur WM","year":"2008","journal-title":"Proc. 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