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However, until now there has been no validated alternative <jats:italic>in vitro<\/jats:italic> method. In this work, 400 compounds used in the cosmetic industry were selected to cover the greatest diversity of structures, biological activities and sensitizing potential. These molecules were submitted to a series of tests aimed at reproducing essential steps in sensitization and to distinguish between sensitization and irritations, i.e., transcutaneous permeation (factor A), haptenation (factor B), sensitization cytokines production (factor C) and acute toxicity (factor D). The transcutaneous diffusion was measured on human skin explants using Franz cells. Haptenation was tested in solution on human serum albumin. Sensitization cytokine production was investigated by measurement of interleukin\u201018 release by keratinocytes. Acute toxicity was determined using an 3\u2010(4,5\u2010Dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide<jats:sup>75<\/jats:sup> cell viability test. As only sufficiently stable, soluble and detectable compounds are usable, 33, 72, 68 and 68 molecules were finally tested on factors A, B, C and D, respectively, and 32 were completely screened by the four factors. The individual correlation of the four factors with the reference <jats:italic>in vivo<\/jats:italic> tests was limited but the combination of these factors led to a correlation between <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> assays of 81.2% and the safety of the test (risk of false negative) reached 96.8%. The techniques employed are simple and inexpensive and this model of four tests appears as a promising technique to evaluate <jats:italic>in vitro<\/jats:italic> the skin sensitization potential of unknown molecules. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.2986","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T11:32:53Z","timestamp":1391513573000},"page":"48-58","source":"Crossref","is-referenced-by-count":6,"title":["Development of a multiparametric <i>in vitro<\/i> model of skin sensitization"],"prefix":"10.1002","volume":"35","author":[{"given":"Muriel","family":"Guyard\u2010Nicod\u00e8me","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology Signals and Microenvironment (LMSM), EA 4312 University of Rouen  55 rue Saint Germain F\u201027000 Evreux France"},{"name":"Hygiene and Quality of Poultry and Pork Products Unit Ploufragan\/Plouzan\u00e9 Laboratory, ANSES  BP53 F\u201022440 Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloise","family":"Gerault","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Signals and Microenvironment (LMSM), EA 4312 University of Rouen  55 rue Saint Germain F\u201027000 Evreux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Platteel","sequence":"additional","affiliation":[{"name":"Biogalenys SAS  9 rue de Pacy F\u201027930 Miserey France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Peschard","sequence":"additional","affiliation":[{"name":"Sederma  29 rue du Chemin Vert F\u201078612 Le Perray en Yvelines France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried","family":"Veron","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology Signals and Microenvironment (LMSM), EA 4312 University of Rouen  55 rue Saint Germain F\u201027000 Evreux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Mondon","sequence":"additional","affiliation":[{"name":"Sederma  29 rue du Chemin Vert F\u201078612 Le Perray en Yvelines France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svinareff","family":"Pascal","sequence":"additional","affiliation":[{"name":"Biogalenys SAS  9 rue de Pacy F\u201027930 Miserey France"},{"name":"BioAdmetys SAS  9 rue de Pacy F\u201027930 Miserey France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc G. 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