{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:06:14Z","timestamp":1790658374904,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,3,30]],"date-time":"2015-03-30T00:00:00Z","timestamp":1427673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Applied Toxicology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The skin sensitization potential of chemicals has been determined with the use of the murine local lymph node assay (LLNA). However, in recent years public concern about animal welfare has led to a requirement for non\u2010animal risk assessment systems for the prediction of skin sensitization potential, to replace LLNA. Selection of an appropriate <jats:italic>in vitro<\/jats:italic> test or <jats:italic>in silico<\/jats:italic> model descriptors is critical to obtain good predictive performance. Here, we investigated the utility of artificial neural network (ANN) prediction models using various combinations of descriptors from several <jats:italic>in vitro<\/jats:italic> sensitization tests. The dataset, collected from published data and from experiments carried out in collaboration with the Japan Cosmetic Industry Association (JCIA), consisted of values from the human cell line activation test (h\u2010CLAT), direct peptide reactivity assay (DPRA), SH test and antioxidant response element (ARE) assay for chemicals whose LLNA thresholds have been reported. After confirming the relationship between individual <jats:italic>in vitro<\/jats:italic> test descriptors and the LLNA threshold (e.g.\u2009EC3 value), we used the subsets of chemicals for which the requisite test values were available to evaluate the predictive performance of ANN models using combinations of h\u2010CLAT\/DPRA (<jats:italic>N<\/jats:italic> = 139 chemicals), the DPRA\/ARE assay (<jats:italic>N<\/jats:italic> = 69), the SH test\/ARE assay (<jats:italic>N<\/jats:italic> = 73), the h\u2010CLAT\/DPRA\/ARE assay (<jats:italic>N<\/jats:italic> = 69) and the h\u2010CLAT\/SH test\/ARE assay (<jats:italic>N<\/jats:italic> = 73). The h\u2010CLAT\/DPRA, h\u2010CLAT\/DPRA\/ARE assay and h\u2010CLAT\/SH test\/ARE assay combinations showed a better predictive performance than the DPRA\/ARE assay and the SH test\/ARE assay. Our data indicates that the descriptors evaluated in this study were all useful for predicting human skin sensitization potential, although combinations containing h\u2010CLAT (reflecting dendritic cell\u2010activating ability) were most effective for ANN\u2010based prediction. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jat.3105","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T02:59:18Z","timestamp":1427857158000},"page":"1333-1347","source":"Crossref","is-referenced-by-count":56,"title":["Evaluation of combinations of <i>in vitro<\/i> sensitization test descriptors for the artificial neural network\u2010based risk assessment model of skin sensitization"],"prefix":"10.1002","volume":"35","author":[{"given":"Morihiko","family":"Hirota","sequence":"first","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. Ltd.  2\u20102\u20101 Hayabuchi, Tsuzuki\u2010ku Yokohama\u2010shi Kanagawa 224\u20108558 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiho","family":"Fukui","sequence":"additional","affiliation":[{"name":"Kanebo Cosmetics Inc.  3\u201028, Kotobukicho 5\u2010chome Odawara Kanagawa 250\u20100002 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Kanebo Cosmetics Inc.  3\u201028, Kotobukicho 5\u2010chome Odawara Kanagawa 250\u20100002 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kurotani","sequence":"additional","affiliation":[{"name":"Kose Corporation  1\u201018\u20104 Azusawa, Itabashi\u2010ku Tokyo 174\u20100051 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyasu","family":"Imai","sequence":"additional","affiliation":[{"name":"Kose Corporation  1\u201018\u20104 Azusawa, Itabashi\u2010ku Tokyo 174\u20100051 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyuki","family":"Fujishiro","sequence":"additional","affiliation":[{"name":"Cosmos Technical Center Co., Ltd.  3\u201024\u20103 Hasune, Itabashi\u2010ku Tokyo 174\u20100046 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiki","family":"Kyotani","sequence":"additional","affiliation":[{"name":"Cosmos Technical Center Co., Ltd.  3\u201024\u20103 Hasune, Itabashi\u2010ku Tokyo 174\u20100046 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinao","family":"Kato","sequence":"additional","affiliation":[{"name":"Nippon Menard Cosmetic Co.  Ltd., 2\u20107, Torimi\u2010cho, Nishi\u2010ku Nagoya 451\u20100071 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Kasahara","sequence":"additional","affiliation":[{"name":"Fujifilm Corporation  210, Nakamura Minamiashigara\u2010shi Kanagawa 250\u20100193 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaharu","family":"Fujita","sequence":"additional","affiliation":[{"name":"Fujifilm Corporation  210, Nakamura Minamiashigara\u2010shi Kanagawa 250\u20100193 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akemi","family":"Toyoda","sequence":"additional","affiliation":[{"name":"Pola Chemical Industries, Inc.  560 Kashio\u2010cho, Totsuka\u2010ku Yokohama 244\u20100812 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Sekiya","sequence":"additional","affiliation":[{"name":"Lion Corporation  100, Tajima Odawara Kanagawa 256\u20100811 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Lion Corporation  100, Tajima Odawara Kanagawa 256\u20100811 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Seto","sequence":"additional","affiliation":[{"name":"P&amp;G Japan K.K.  1\u201017, Koyo\u2010cho Naka, Higashinada\u2010ku Kobe 658\u20100032 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Takenouchi","sequence":"additional","affiliation":[{"name":"Kao Corporation  2606, Akabane, Ichikai\u2010Machi, Haga\u2010Gun Tochigi 321\u20103497"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Ashikaga","sequence":"additional","affiliation":[{"name":"Shiseido Research Center, Shiseido Co. 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